Pregled bibliografske jedinice broj: 6225
RFLP and SSCP of the HGF gene (IV exon PCR product)
RFLP and SSCP of the HGF gene (IV exon PCR product) // First European-American Intensive Course in PCR Based Clinical and Forensic Testing : Laboratory Manual / Burgi, Dean S. (ur.).
Split: Laboratorij za kliničku i molekularnu genetiku, Klinička bolnica, 1997. str. 158-160 (pozvano predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 6225 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
RFLP and SSCP of the HGF gene (IV exon PCR product)
Autori
Topić, Elizabeta ; Žuntar, Irena ; Štefanović, Mario
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
First European-American Intensive Course in PCR Based Clinical and Forensic Testing : Laboratory Manual
/ Burgi, Dean S. - Split : Laboratorij za kliničku i molekularnu genetiku, Klinička bolnica, 1997, 158-160
Skup
First European-American Intensive Course in PCR Based Clinical and Forensic Testing
Mjesto i datum
Split, Hrvatska, 23.09.1997. - 03.10.1997
Vrsta sudjelovanja
Pozvano predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
hepatocyte growth factor; HGF; polymorphysm; PCR-RFLP; PCR-SSCP exon 4th
Sažetak
Hepatocyte growth factor (HGF) is a multifunctional cytokine and a major factor of liver regeneration, regulating the cell growth, development, motility and morphogenesis. Active HGF consists of a (64 kDa) and b (34 kDa) chains linked by disulfide bond. The gene is located on chromosome 7, locus q 11.1-21, and consists of 18 exons and 17 introns of approximately 70 kb. The structure of HGF contains four kringle domains, each of them encoded by two exons. The first and second kringle domain, and hairpin loop are essential for HGF recognition by c-met/HGF receptors, as well as for HGF structural domain. The forth and fifth exons encode for the first structural domain. As mutations in functionally and biologically relevant domains lead to a pathologic process, polymorphism studies in healthy subjects and patients with liver diseases, and determination of their association with the occurrence of disease are of utmost importance. The polymorphism of DNA fragment obtained by polymerase chain reaction (PCR) can be determined by two established methods, i.e. restriction fragment length polymorphism (RFLP) and single-strand conformation polymorphism (SSCP). Here we described the PCR-RFLP and PCR-SSCP protocol for the polymorphism analysis of exon 4th of the HGF gene.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
