Naslov
Epigenetic variation in the absence of genetic variation contribute to adaptation to specific eco-geographical conditions in endemic Lilium bosniacum

Autori
Biruš, Ivan ; Bečeheli, Ivona ; Šatović, Zlatko ; Vičić, Vedrana ; Muratović, Edina ; Pustahija, Fatima ; Šiljak-Yakovlev, Sonja ; Zoldoš, Vlatka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
3rd Congress of Croatian geneticists with international participation : Book of abstracts / Franekić, Jasna ; Garaj-Vrhovac, Verica - Sveti Ivan Zelina, 2012, 66-66

Skup
Congress of Croatian geneticists with international participation (3 ; 2012)

Mjesto i datum
Krk, Hrvatska, 13.05.2012. - 16.05.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
epigenetics; adaptation; Lilium bosniacum

Sažetak
In plants, which are sessile organisms, epigenetics provides a pronounced flexibility and plasticity to a genome in response to environmental stress. Natural variation in DNA methylation profiles between plant populations, ecotypes and accessions has been well evidenced and correlated with diverse ecological factors. A very small population of endemic Lilium bosniacum was found at the microsite Kladanj (BIH) growing under unusual eco-geographical conditions for this species, which contain several types of abiotic stress. In this study we analysed genetic and epigenetic structure of this and the control population by Random Amplified Polymorphic DNA (RAPD) and Coupled Restriction Enzyme Digestion and Random Amplification (CRED-RA), respectively. The RAPD data revealed highly genetic similarity between the two populations of L. bosniacum and methylation sensitive RAPD (CRED-RA) revealed different DNA methylation status between and within the two populations. Shannon’s information index, as a measure of both RAPD and CRED-RA diversity, suggested a lower genetic and higher epigenetic diversity in the population from the microsite Kladanj. In addition, pairwise genetic and epigenetic distances revealed lower genetic and nonetheless higher epigenetic distances among individuals belonging to the population Kladanj in comparison to those of the control population. This result could suggest that the population of Kladanj, following a bottleneck, lost some portion of the pre-existing genetic variation due to selection and/or drift, but, simultaneously, being under environmental stress, epigenetic plasticity led to a higher diversity of DNA methylation patterns. Non- significant correlation between genetic and epigenetic distances indicates that the epigenetic changes were mostly independent from variations at the DNA level. A higher proportion of molecular variance attributable to between-population component, as revealed on epigenetic level in comparison to genetic, seems to support the hypothesis of environmental impact on the epigenome as causing much more rapid change than 'classical' evolutionary processes at the genome level. Our study demonstrates an important association of DNA methylation change and environmental factors.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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