Naslov
Renal distribution of ganglioside GM3 in rat models of type 1 and type 2 diabetes

Autori
Novak, Anela ; Režić Mužinić, Nikolina ; Čikeš Čulić, Vedrana ; Božić, Joško ; Tičinović Kurir, Tina ; Ferhatović, Lejla ; Puljak, Livia ; Markotić, Anita

Izvornik
Journal of physiology and biochemistry (1138-7548) 69 (2013), 4; 727-735

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
rat; diabetes mellitus; streptozotocin; kidney; GM3

Sažetak
Ganglioside GM3 is particularly abundant in the kidney tissue and are thought to play an important role in the maintenance of the charge-selective filtration barrier of glomeruli. Altered expression of ganglioside GM3 was pathologically related with glomerular hypertrophy occurring in diabetic human and rat kidneys. Considering the role of GM3 ganglioside in kidney function, the aim of this study was to determine the difference in expression of GM3 ganglioside in glomeruli and tubules using immunofluorescence microscopy both in rat models of type 1 and type 2 diabetes mellitus. Diabetes was induced with streptozotocin (55 mg/kg for type 1 diabetes and 35 mg/kg for type 2 diabetes) injection to male Sprague-Dawley rats which were fed with normal pellet diet (type 1 diabetes) or high-fat diet (type 2 diabetes). Rats were sacrified two weeks after diabetes induction, frozen renal sections were stained with primary antibody GM3(Neu5Ac) and visualized by secondary antibody coupled with Texas-Red. In addition renal ganglioside GM3 were analysed by high performance thin layer chromatography followed by GM3 immunostaining. Immunofluorescent microscopy detected 1.7 fold higher GM3 expression in tubules and 1.25 fold higher GM3 in glomeruli of type 1 diabetes mellitus compared to control group. Type 2 diabetes mellitus rats showed slight GM3 increase in whole kidney, unchanged GM3 in glomeruli, but significant higher GM3 expression in tubules, compared to control animals. Taking into consideration increased tubular GM3 content in both types of diabetes, we could hypothesize the role of GM3 in early pathogenesis of diabetic nephropathy.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija

POVEZANOST RADA