Naslov
Effect of the Oil Industry High Density Brines on Tissues of Planorbarius corneus L.

Autori
Mažuran, Neda ; Klobučar, Goran ; Kovačević, Goran

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Croatian Microscopy Symposium/Hrvatski mikroskopijski simpozij Book of Abstracts/Knjiga sažetaka / Gajović, Andreja ; Tomašić, Nenad - Zagreb : Hrvatsko mikroskopijsko društvo, 2012, 69-70

ISBN
978-953-57138-1-4

Skup
Croatian Microscopy Symposium/Hrvatski mikroskopijski simpozij

Mjesto i datum
Pula, Hrvatska, 16.11.2012. - 17.11.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
oil industry high density brines; CaCl2; CaBr2; Planorbarius corneus L.; tissue damage investigation

Sažetak
High density brines are concentrated salt solutions which are regularly used to control the hydrostatic pressure in oil wells during special operations in exploration and production of natural gas and crude oil [1]. Calcium, sodium, potassium, magnesium and zinc chloride and bromide are most usually used as workover high density fluids, in quantity of 5 – 20 m3 per well [2]. Various concentrations of high density brines are left in the mud pits nearby the drilling site as waste and may pollute fresh and ground waters by spilling over and drain off through the ground. Investigating the effects of that kind of pollution was necessary, so a more extensive laboratory research of the toxic effects of high density brines on several test organisms (Lemna minor L., Daphnia magna L. and Allium test) was carried out before. The research results of the effect of sublethal concentrations of calcium chloride and calcium bromide and their mixtures 1:1 on the survival, growth and fecundity of the freshwater snail Planorbarius corneus L. showed a considerable dose-response decline of the survival, growth and fecundity [3]. In this study the effect of lethal concentrations of calcium chloride and calcium bromide (5985 ppm CaCl2 and 7939 ppm CaBr2) on the tissues of Planorbarius corneus L. was investigated. Histological samples were prepared by fixing the tissues with Bouin's solution (saturated picric acid, glacial ethanoic acid and formalin), imbedding in paraffin and sectioning between 6-8 μm. Samples were then stained using hemalaun-eosin [4]. The prepared samples showed no obvious histopathological changes except widening of hemolymphatic area in some organs (excretory organ, mantle cavity, osphradium, salivary gland) in comparison to the control (Figures 1 and 2). It was concluded that hyperosmotic medium caused a higher hemolymph viscosity and slower flow thus accumulating hemolymph in the hemolymphatic area and causing observed expansions.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA