Naslov
Detection of cathepsin D in ewe milk by Western Blotting method

Autori
Dolenčić Špehar, Iva, Martinković, Franjo ; Havranek, Jasmina ; Marinculić Albert ; Tudor Kalit, Milna ; Kalit, Samir

Izvornik
Mljekarstvo (0026-704X) 63 (2013), 1; 36-41

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, prethodno priopćenje, znanstveni

Ključne riječi
milk; cathepsin D; ewe milk; Western Blotting

Sažetak
Milk contains about 70 indogene enzymes, while only twenty of them were researched. One of the less explored is the milk enzyme cathepsin D, proteolytic enzyme located in the lysosomes, which are an integral part of the somatic cells whose number varies depending on the animal’s health. Unlike cow’s milk, in ewe’s milk was not determined the presence of cathepsin D, which could affect on the production of dairy products especially cheese, which are traditionally produced in Croatia and Mediterranean. This paper shows the presence of cathepsin D and its forms in ewe's milk by modified Western Blotting method. The analysis confirmed the presence of procathepsin D, mature and heavy chain of cathepsin D. Light chain cathepsin D was not detected.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina, Poljoprivreda (agronomija), Biotehnologija

POVEZANOST RADA