Pregled bibliografske jedinice broj: 618061
Ikaros family transcription factors expression in rat thymus : detection of impaired development
Ikaros family transcription factors expression in rat thymus : detection of impaired development // International journal of immunopathology and pharmacology, 25 (2012), 4; 893-900 doi:10.1177/039463201202500407 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 618061 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Ikaros family transcription factors expression in rat thymus : detection of impaired development
Autori
Paradžik, Mladen ; Novak, Sanja ; Mokrović, Gordana ; Bordukalo Nikšić, Tatjana ; Heckel, Darko ; Stipić, Jagoda ; Pavičić Baldani, Dinka ; Čičin-Šain, Lipa ; Antica, Mariastefania
Izvornik
International journal of immunopathology and pharmacology (0394-6320) 25
(2012), 4;
893-900
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
transcription factors ; Ikaros ; Aiolos ; Helios ; rat thymus ; development
Sažetak
The expression of Ikaros family transcription factors and consequently their signalling pathway is limiting for hematopoietic and lymphocyte development in mice and human. Due to their importance, these transcription factors are highly homologous between species. As an initial approach to examining the possible involvement of Ikaros transcription factors in pathogenesis of rat lymphoid development, we analyzed the expression of all known Ikaros family members, Ikaros, Aiolos, Helios, Eos and Pegasus in the rat thymus. We established a semi-quantitative RT-PCR to detect mRNA of each transcription factor. For the first time we give evidence of the expression of Ikaros family transcription factors in the rat thymus. Further, we evaluated whether their mRNA expression was succumbed to changes when the rats were exposed to ethanol, as a known debilitating agent during development. Therefore we analyzed the thymus of adult rats whose mothers were forced to drink ethanol during gestation, to detect possible changes in thymus mRNA expression levels of Ikaros, Aiolos, Helios, Eos and Pegasus. We found that rats prenatally exposed to ethanol show a slightly higher expression of Ikaros family transcription factors in the adult thymus when compared to control rats, but these differences were not statistically significant. We further studied the distribution of the major lymphocyte subpopulations in the rat thymus according to CD3, CD4 and CD8 expression by four color flow cytometry. We found a higher incidence of CD3 positive cells in the double positive, CD4+CD8+ thymic subpopulation of rats prenatally exposed to ethanol when compared to non-exposed animals. Our findings indicate that ethanol exposure of pregnant rats might influence the development of CD3 positive cells in the thymus of the offspring but this result should be further tackled at the level of transcription factor expression.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Temeljne medicinske znanosti, Veterinarska medicina
POVEZANOST RADA
Projekti:
098-0982913-2332 - Molekularne interakcije u diferencijaciji limfocita (Antica, Mariastefania, MZOS ) ( CroRIS)
098-1081870-2397 - Serotonergični mehanizmi u alkoholizmu (Čičin-Šain, Lipa, MZOS ) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb,
Klinički bolnički centar Zagreb
Profili:
Sanja Novak
(autor)
Dinka Pavičić Baldani
(autor)
Mladen Paradžik
(autor)
Tatjana Bordukalo Nikšić
(autor)
Lipa Čičin-Šain
(autor)
Jagoda Stipić
(autor)
Mariastefania Antica
(autor)
Gordana Mokrović
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE