Naslov
Diatom Polysaccharides: Extracellular Production, Isolation and Molecular Characterization

Autori
Urbani, Ranieri ; Paola, Sist ; Pletikapić, Galja ; Mišić Radić, Tea ; Svetličić, Vesna ; Žutić, Vera

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, pregledni

Knjiga
The Complex World of Polysaccharides

Urednik/ci
Desiree Nedra, Karunaratne,

Izdavač
IntechOpen

Grad
Lahti

Godina
2012

Raspon stranica
345-367

ISBN
098-0982934-2744

Ključne riječi
polysaccharides ; extracellular polysaccharide ; viscometry ; DLS ; atomic force microscopy ; marine gel network ; Northern Adriatic macroaggregates

Sažetak
This chapter aims to give an overview on the isolation procedures and techniques for macromolecular characterization of extracellular polysaccharides from laboratory cultures of selected diatom species and from native marine aggregates. The emphasis is given to the macromolecular behavior of isolated polysaccharide in solution and their propensity to aggregate and to form highly structured systems like gels. This was made possible by developing protocols and procedures for isolation/separation and application of physico-chemical techniques like laser light scattering and viscosimetry to marine and culture samples. A separate section is devoted to atomic force microscopy, the most versatile nanotechnology platform widely used in polymer biophysics and diatom nanotechnology. The first force spectra experiments conducted on isolated polysaccharide fibrils and on marine gel polysaccharide network are presented together with recently published works on high resolution imaging. Diatom exopolysaccharide force spectroscopy and interactions with nanoparticles are identified as future prospective contributing to the growing field of nanoecology and for rational design of polysaccharide gels with desired properties.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Biotehnologija

POVEZANOST RADA