Naslov
Applicability of liquid chromatography with time-of-flight mass spectrometric detection for determination of cyanuric acid in soil

Autori
Stipičević, Sanja ; Mendaš, Gordana ; Vuletić, Marko ; Fingler, Sanja ; Drevenkar, Vlasta

Izvornik
Croatica chemica acta (0011-1643) 86 (2013), 1; 95-102

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cyanuric acid; soil; liquid chromatography; time-of-flight mass spectrometry; soil matrix interferences

Sažetak
Cyanuric acid (2, 4, 6-trihydroxy-1, 3, 5-triazine, CA) is a highly polar and hydrophilic degradation product of symmetric triazine herbicides, fairly stable under environmental conditions. We investigated the applicability of reversed-phase liquid chromatography coupled to time-of-flight mass spectrometry (LC-ToF-MS) for qualitative and quantitative determination of CA in soils of different origin and pedological characteristics. CA was ultrasonically extracted from soils either with a mixture of acetone and n-hexane (volume ratio = 2 : 1) or with methanol and analysed on a LiChrospher 100 CN column with ψ(acetonitrile, water) = 50 : 50 as the mobile phase for isocratic elution. The efficiency and selectivity of LC-ToF-MS determination, using the electrospray ionisation in negative mode and selective monitoring of deprotonated CA molecule (m/z 128), was evaluated with respect to injection volume, extracting solvent, and possible soil matrix interferences. The advantage of LC-ToF-MS over conventional HPLC with UV diode array detection was the high ion detection selectivity minimising the interferences of coeluting species and the unambiguous identification of CA in soil extracts by accurate mass measurements of deprotonated molecule.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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