Naslov
Epigenetic regulation of surface N-glycosylation in HeLa cells

Autori
Barišić, Darko

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Prirodoslovno-matematički fakultet

Mjesto
Zagreb

Datum
11.10

Godina
2012

Stranica
50

Mentor
Zoldoš, Vlatka ; Horvat, Tomislav

Neposredni voditelj
Horvat, Tomislav

Ključne riječi
DNA methylation; epigenetic inhibitors; HeLa cell line; histone acetylation; protein N-glycosylation; surface N-glycome

Sažetak
N-glysoylation is a covalent modification of a protein important for its proper folding and function. Since cell surface represents the first front in cell-to-cell communication and communication between the cell and its environment, surface glycosylation can reflect changes in the environment as well as modify cellular signaling, recognition and adaptive changes in reaction to pathogens and factors of immune system. Changes in expression of glyco-genes through variety of epigenetic mechanisms represent one aspect of regulating glycan synthesis and protein glycosylation. To address the questions regarding the role of epigenetic information in N-glycoslylation, we studied changes in surface N-glycome of HeLa cells upon treatment with epigenetic inhibitors of DNA methyltransferases and histone deacetylasesas as well as reversibility of altered N-glycan profile. Since epigenetic inhibitors are currently explored as therapeutics in treatment of cancer, the presented work contributes to our understanding of their efficiency in altering the N-glycan profile of cancer cells.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

Napomena
Horvat Tomislav je pri izradi ovog diplomskog rada bio pomoćni voditelj.

POVEZANOST RADA