Pregled bibliografske jedinice broj: 609810
High Resolution Structure of the Manganese Derivative of Insulin
High Resolution Structure of the Manganese Derivative of Insulin // Croatica chemica acta, 85 (2012), 4; 435-439 doi:10.5562/cca2108 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 609810 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
High Resolution Structure of the Manganese Derivative of Insulin
Autori
Prugovečki, Biserka ; Pulić, Ivana ; Toth, Martina ; Matković-Čalogović, Dubravka
Izvornik
Croatica chemica acta (0011-1643) 85
(2012), 4;
435-439
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
insulin derivative; manganese; X-ray structure
Sažetak
The structure of the human manganese derivative of insulin was determined using the single crystal X-ray diffraction method. Single crystals were grown by the hanging drop vapour diffusion crystallization method using zinc-free insulin and manganese(II) sulphate monohydrate in citrate buffer at pH 6.4. It crystallizes in the trigonal system in the space group R3 with the unit cell parameters: a = b = 81.70 Å and c = 33.73 Å. There are two manganese ions per insulin hexamer in the human manganese-insulin derivative. The manganese ions lie on the three-fold crystallographic axis on opposite sides of the hexamer. Both manganese ions are octahedrally coordinated by three N2 atoms from the imidazolyl side chains of three symmetry-related HisB10/HisD10 and three oxygen atoms of three symmetry-related water molecules. The investigated human manganese-insulin derivative adopts the T6 conformation.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
POVEZANOST RADA
Projekti:
119-1193079-1084 - Strukturno istraživanje bioloških makromolekula metodom rentgenske difrakcije (Matković-Čalogović, Dubravka, MZOS ) ( CroRIS)
Ustanove:
Prirodoslovno-matematički fakultet, Zagreb
