Naslov
ADH based NAD+ regeneration in a microreactor

Autori
Šalić, Anita ; Ivanković, Mia ; Ferk, Elvira ; Zelić, Bruno

Izvornik
Journal of chemical technology and biotechnology (0268-2575) 88 (2013), 9; 1721-1729

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
NAD+ ; coenzyme regeneration ; microreactor ; mathematical modelling

Sažetak
The coenzyme NAD+ (nicotinamide adenine dinucleotide) is commonly used in biocatalytic oxidations catalysed by the enzyme alcohol dehydrogenase (ADH). As the price of the coenzyme NAD+ is extremely high, it is essential to regenerate the reduced form of the coenzyme back into the oxidized form. In this paper the regeneration of the coenzyme NAD+ was carried out in a microreactor by reversible oxidation of ethanol to acetaldehyde with the ADH enzyme. A 100 % conversion of NADH was achieved for a residence time of just τ = 0.8 s when the concentration of acetaldehyde was in excess (ci, NADH = 5.5 mmol/dm3, ci, acetaldehyde = 44 mmol/dm3, γi, ADH = 0.2 g/dm3, 75 mmol/dm3 glycine-pyrophosphate buffer pH = 9 ; T = 25 °C). A 2D mathematical model for the description and prediction of microreactor performance was developed. Model simulations were validated using set of data from independent experiments. Due to high conversions that were obtained for short residence times, microreactors could be considered as good and efficient toll for coenzyme regeneration.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo

Napomena
Ovaj se tekst temelji na radu koji je financirala Hrvatska zaklada za znanost.

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