Naslov
Clinical significance of NKT and Treg cells in autoimmune thyroid disease during pregnancy

Autori
Mrakovčić-Šutić, Ines ; BogoviĆ-Crnčić, Tatjana ; Bulog, Aleksandar ; Mićović, Vladimir ;

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
European Congress of Immunology-Glasgow 2012.

Mjesto i datum
Glasgow, Ujedinjeno Kraljevstvo, 05.09.2012. - 08.09.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
autoimmune thyreoid disease; NKT cells; Treg cells

Sažetak
Purpose/Objective: NKT cells represent a unique sublineage of innate lymphocytes, that share the properties of natural killer cells and conventional T cells. Autoimmune thyroid dysfunction (ATD), which comprises two main clinical entities: Graves' disease and Hashimoto thyroiditis, often affect women of reproductive age. In a healthy pregnancy predominant is Th2 over Th1 immunity, which explains the improvement of autoimmune disease during pregnancy, while after birth due to changes in Th1/Th2 ratios often leads to deterioration of ATD. NKT and Tregs seem to play an important part in mediating maternal tolerance to fetus. Although many researches have been done in the field of thyroid autoimmunity, very few studies investigated the role of innate immunity in AITD during human pregnancy and in the postpartal period. Materials and Methods: We investigated the presence of ATD in pregnant and postpartum period in women with hormonal status determination, the titer of thyroid antibodies and auto antibodies and compared them with healthy pregnant women and subjects’ postpartum and not pregnant women. After intracellular and surface staining using flow cytometry, we analyzed the phenotype and cytolytic potential of isolated peripheral blood mononuclear cells of pregnant women and postpartum women, and not pregnant women. Results: Total in 25% of pregnant women and 41.5% of postpartum women have demonstrated the presence of thyroid autoimmunity. The cells of innate immunity: NKT and Tregs are elevated in healthy pregnancies and in pregnancies with hypo-and hyperthyroidism, as well as postpartum in all experimental groups. NKT cells are reduced in pregnant women with positive antibodies indicating a decrease in NKT cell protective effect of pregnancy under the action of antibodies. Total perforin was decreased in healthy women, but higher in patients with positive antibodies, hypo-and hyperthyroidism and postpartum indicating the increased cytolytic potential of these cells in autoimmune process. Conclusions: Pregnancy and the postpartum period influence the function of the thyroid gland. In the presence of thyroid autoimmunity changes are more pronounced, especially postpartum. Apart from pregnancy and postpartum period influence the course of ATD and thyroid autoimmunity affects thyroid function in pregnancy and the postpartum period.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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