Naslov
An Overexpression of Icam-1 in Mild Hyperhomocysteinemia and Hyperglycemia − A Study of Antidiabetics Administration Effect

Autori
Bačun, Tatjana ; Belovari, Tatjana ; Včev, Aleksandar ; Mihaljević, Ivan ; Hanich, Toni ; Fijačko, Vladimir ; Glavaš-Obrovac, Ljubica

Izvornik
Croatica chemica acta (0011-1643) 85 (2012), 2; 185-191

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cell adhesion molecules; human aortic endothelial cells; mild hyperhomocysteinemia; mild

Sažetak
Elevated plasma homocysteine is connected to atherosclerosis and increased cerebrovascular and ischemic heart disease especially in the patients with type 2 diabetes. Effects of metformin (10 μg mL−1), insulin (1 mUI mL−1), and their combination administration in condition of mild hyperhomocysteinemia (30 μmol dm−3) and hyperglycemia (12 mmol dm−3) on human aortic endothelial cells (HAEC) was investigated. HAEC were cultured 4 h in a medium with homocysteine (7 and 30 μmol dm−3) and glucose (5.5 and 12.0 mmol dm−3). E-selectin, intercellular adhesion molecule-1 (ICAM-1), and vascular adhesion molecule-1 (VCAM-1) expressions were analysed by flow-cytometry. Controls were CAMs expression on HAEC in medium with physiological concentration of homocysteine and glucose. HAEC, incubated additionally with 30 μmol dm−3 homocysteine, increased expression of ICAM-1 for 65 % (p = 0.07) and decreased of E-selectin for 30 % (p = 0.07) and VCAM-1 for 13 % (0.06). In condition of mild hyperhomocysteinemia and hyperglycemia there was a statistically significant increase of ICAM- 1 expression (for 22 %, p = 0.04), while administration of metformin and insulin did not statistically significantly influence adhesion molecules expression. Observed significant overexpression of ICAM-1, leads us to conclude that combination of mild hyperhomocisteinemia and hyperglycemia could have significant influence on development of inflammation and atherosclerosis in diabetic patients.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA