Pregled bibliografske jedinice broj: 600396
Stress tolerance assays and genetic diversity of Sinorhizobium meliloti field population
Stress tolerance assays and genetic diversity of Sinorhizobium meliloti field population // 4th International Congress EUROSOIL 2012 Soil Science for the Benefit for the Mankind and Environment - Book of Abstracts
Bari, Italija, 2012. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 600396 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Stress tolerance assays and genetic diversity of Sinorhizobium meliloti field population
Autori
Sikora, Sanja ; Huić Babić, Katarina ; Blažinkov, Mihaela ; Rajnović, Ivana ; Bazzicalupo, Marco
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
4th International Congress EUROSOIL 2012 Soil Science for the Benefit for the Mankind and Environment - Book of Abstracts
/ - , 2012
Skup
4th International Congress EUROSOIL 2012 Soil Science for the Benefit for the Mankind and Environment
Mjesto i datum
Bari, Italija, 02.07.2012. - 06.07.2012
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
nitrogen fixation; rhizobia; Sinorhizobium meliloti; indigenous strains; stress tolerance; genetic diversity
Sažetak
The studying of rhizobial biodiversity opens up the possibility to preserve and maybe to exploit some indigenous strains with hidden symbiotic or ecological potential. In order to improve the beneficial effect of alfalfa inoculation it is important to characterize the indigenous strains and to obtain information about actual composition of rhizobial field population. The main aim of the present study was to characterize indigenous rhizobia associated with alfalfa (Medicago sativa L.) in different regions of Croatia. Over 250 isolates were obtained from root nodules of alfalfa. To study their diversity and characterize them in relation to environmental conditions of their soils of origin, a polyphasic approach was used. Stress tolerance assays revealed significant variations in pH tolerance while almost all isolates showed similar tolerance to elevated salt concentrations and growth temperatures. PCR-RFLP of 16S rDNA revealed most of isolates to be closely related to S. meliloti. However, several isolates produced slightly different RFLP pattern from S. meliloti type strain and other isolates. Dendrogram derived from AFLP profiles revealed considerable genetic diversity among S. meliloti isolates. Only a few strains were identical or nearly identical to each other. The strains originated from the same region were mostly grouped within the same cluster. Better understanding of rhizobial ecology and selection of locally adapted and genetically defined strains as well as their targeted application is one among many approaches to improve the nitrogen fixation efficiency and crop productivity.
Izvorni jezik
Engleski
Znanstvena područja
Poljoprivreda (agronomija)
POVEZANOST RADA
Projekti:
178-1780692-0558 - Korisne mikrobne zajednice u održivom uzgoju leguminoza (Sikora, Sanja, MZOS ) ( CroRIS)
Ustanove:
Agronomski fakultet, Zagreb
Profili:
Mihaela Blažinkov
(autor)
Ivana Rajnović
(autor)
Katarina Huić Babić
(autor)
Sanja Sikora
(autor)