Naslov
ASSESSMENT OF NITROFURANTOIN AS ECOTOXICANT

Autori
SAUERBORN KLOBUČAR, Roberta ; BROZOVIĆ, Anamaria ; ŠTAMBUK, Anamaria ; ŠRUT, Maja ; TOPIĆ POPOVIĆ, Natalija ; STRUNJAK PEROVIĆ, Ivančica ; Jadan, Margita ; Barišić, Josip ; Čož-Rakovac, Rozelindra

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
ARCHIVES OF INDUSTRIAL HYGIENE AND TOXICOLOGY: Abstracts of the 4th Croatian Congress of Toxicology (CROTOX 2012) / Nevenka Kopjar - Zagreb : Denona, 2012, 30-30

Skup
4th Croatian Congress of Toxicology (CROTOX 2012)

Mjesto i datum
Zagreb, Hrvatska, 02.10.2012. - 05.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
antibiotic; aquatic environment; cytotoxicity; genotoxicity; growth inhibition

Sažetak
Antibiotic nitrofurantoin (NF) and its derivatives are used in veterinary and human medicine. The impact of NF on the aquatic environment is largely unknown and its ecotoxicity is still under investigation due to confl icting opinions on its use. Increasing evidence suggests that NF can cause various adverse effects in living cells. In this investigation, we used three different fi sh cell lines (PAC2, PLHC-1, R1) to evaluate cytotoxicity of NF towards fi sh. PAC2 cells were shown to be the most sensitive to NF in an MTT colorimetric assay, exhibiting an EC50 of 15 μmol L-1 (3.57 mg L-1) NF. Furthermore, we determined DNA damage and apoptosis in PAC2 cell line depending on the concentration and duration of treatment. We also determined growth inhibition of unicellular algae Scenedesmus subspicatus and mutagenic action towards Salmonella strains (TA98 and TA100) using classic Ames test. A signifi cant decrease in the growth of unicellular algae after 24 h exposure to NF (1 μmol L-1, 10 μmol L-1, 30 μmol L-1, 100 μmol L-1, 200 μmol L-1) was observed. Mutagenicity test responses revealed a large number of revertants following exposure to NF. These results indicate a substantial risk of exposure to NF for the health of aquatic ecosystems

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija)

POVEZANOST RADA