Naslov
Ex vivo evaluation of genotoxic effects of four dental adhesives on human leukocytes

Autori
Tadin, Antonija ; Galić, Nada ; Želježić, Davor ; Mikelić Vitasović, Branimira ; Marović, Danijela ; Kovačić, Ivan

Izvornik
Journal of Dental Sciences (1991-7902) 8 (2013), 1; 37-43

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
biocompatibility ; comet assay ; dental adhesives ; genotoxicity ; leukocytes

Sažetak
The use of dental adhesives in everyday dental practice has raised questions about their biological safety. Their biocompatibility is a relevant aspect of the clinical success of these materials. The objective of this study was to evaluate the genotoxicity of dental adhesives ex vivo using a cytogenetic assay. Four materials (AdheSE, G-Bond, Excite, and Adper Single Bond 2) were tested on human peripheral blood leukocytes using a comet assay. Prepared materials were eluted in a saline solution for 1 h, 1 day, and 5 days. The comet assay was used to evaluate primary DNA damage by measuring the tail length and tail intensity. A Kruskall-Wallis non-parametric test was used for the statistical analysis, with the significance level set to p < 0.05. None of the tested dental adhesives revealed a statistically significant increase in the tail length or tail intensity in treated leukocytes, independent of the applied dilution, elution duration, and polymerization form. A slight increase in the tail length and intensity of DNA molecules was observed after 1 and 5 days of the elution period at the lowest dilution (1: 102) for all tested adhesives, only in their non-polymerized form ; however, these results were not statistically significant. Under the conditions used in this study, all adhesives had acceptable biocompatibility in terms of genotoxicity.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

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