Naslov
Influence of selenium pre-treatment on essential element content and lipid peroxidation in suckling rats exposed to inorganic mercury

Autori
Orct, Tatjana ; Blanuša, Maja ; Vihnanek Lazarus, Maja ; Sekovanić, Ankica ; Jurasović, Jasna ; Piasek, Martina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 4th Croatian Congress of Toxicology (CROTOX 2012) ; u: Arhiv za higijenu rada i toksikologiju 63 (2012) (S2) / Želježić, Davor - , 2012

Skup
4th Croatian Congress of Toxicology with international participation

Mjesto i datum
Primošten, Hrvatska, 02.10.2012. - 05.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
essential micronutrients; mercury exposure; selenium oral supplementation; TBARS assay

Sažetak
Concentrations of copper, zinc, iron, and manganese in the body tissues reflect the nutritional status of laboratory animals. The changes in these essential metals may reflect nutritional deficit or interaction with toxic metals and metalloids. Lipid peroxidation can serve as a biomarker of cellular oxidative stress and has been observed after increased mercury Hg2+ or selenium Se4+ exposure. We investigated the effect of selenium oral supplementation together with oral mercury exposure on the level of essential metals and lipid peroxidation in tissues of neonatal rats. Suckling 7-day-old Wistar rats were pre-treated with selenium (Na2SeO3, 0.51 mg Se/kg b.wt a day) during three days. Following four days, equimolar amounts of mercury (HgCl2, 1.29 mg Hg/kg b.wt a day) were given together with selenium. Pups were euthanized 24 h after the last dose and their liver, kidneys, and brain were dissected and tissue prepared for the analyses. Copper and zinc were measured by atomic absorption spectrometry after digestion with nitric acid. Amount of lipid peroxides (determined by malondialdehyde, MDA) in tissue homogenates was quantified using the thiobarbituric acid reactive substances (TBARS) assay. The HgCl2 exposure alone increased concentrations of Cu and Zn in kidneys while oral supplementation with Na2SeO3 completely prevented this increases. Exposure to mercury significantly increased TBARS production in hepatic and brain tissue preparations ; this effect was only partially prevented by oral selenium supplementation. To conclude, our results show that cautious oral selenium supplementation could prevent the toxic effects of inorganic mercury in mammals in the very early age.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Javno zdravstvo i zdravstvena zaštita

Napomena
Poster presentation P-22

POVEZANOST RADA