Naslov
Urine ochratoxin A and ochratoxin alpha in pregnant women from Croatia

Autori
Klapec, Tomislav ; Šarkanj, Bojan ; Banjari, Ines ; Strelec, Ivica ; Marinović Vitez, Novela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 4th Croatian Congress of Toxicology (CROTOX 2012) Primošten, Croatia u Arhiv za higijenu rada i toksikologiju, Vol. 63, Supplement 2 / Želježić, Davor - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2012, 43-43

Skup
4th Croatian Congress of Toxicology (CROTOX 2012)

Mjesto i datum
Primošten, Hrvatska, 02.10.2012. - 05.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
detection of ochratoxins ; HPLC-FLD method ; mean daily dietary intake ; pregnancy

Sažetak
This study determined exposure of pregnant women to ochratoxin A (OTA). Forty samples of fi rst- void urine samples from Croatian women in the third trimester of pregnancy were analysed for OTA and its major metabolite ochratoxin alpha (OTα). The subjects fi lled a short food frequency questionnaire (FFQ). Analysis was performed by HPLC-FLD following liquid-liquid extraction. All samples were subjected in parallel to enzymatic treatment (β-glucuronidase / aryl sulfatase) to release OTA and OTα from the conjugates. The median urinary levels of OTA and OTα before treatment were 0.02 ng mL-1 [range: (nd to 1.07) ng mL-1] and 0.16 ng mL-1 [(nd to 1.86) ng mL-1]. The concentrations after enzyme hydrolysis were 0.02 ng mL-1 [(nd to 1.11) ng mL-1] and 1.18 ng mL-1 [(0.11 to 7.57) ng mL-1]. While OTα levels increased signifi cantly following enzymatic treatment, evidence for OTA conjugation was weak. The ratio of urinary OTα medians after and before hydrolysis was 1.5 times higher than previously reported for nonpregnant female subjects, possibly indicating upregulated metabolism and/or elimination of mycotoxins and metabolites in pregnancy. The mean daily dietary OTA intake calculated from FFQs [(1.08±0.57) ng kg-1 b. w.] was well below the provisional tolerable daily intake and the greatest contributors to intake were cereal products, fruit juices, chocolate, and coffee.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA