Pregled bibliografske jedinice broj: 596329
Decrease of bovine acetylcholinesterase activity after the exposure to triazole pesticides
Decrease of bovine acetylcholinesterase activity after the exposure to triazole pesticides // Book of Abstracts of the XXV International Conference Genetic Days / Wierzbicki, H., Zatoń-Dobrowolska, M. (ur.).
Wrocław: Department of Genetics, Wrocław University of Environmental and Life Sciences, 2012. str. 111-111 (poster, međunarodna recenzija, sažetak, znanstveni)
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Naslov
Decrease of bovine acetylcholinesterase activity after the exposure to triazole pesticides
Autori
Kolesarová, Viera ; Šiviková, Katarina ; Dianovský , Ján ; Šinko , Goran ; Kovarik , Zrinka
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of Abstracts of the XXV International Conference Genetic Days
/ Wierzbicki, H., Zatoń-Dobrowolska, M. - Wrocław : Department of Genetics, Wrocław University of Environmental and Life Sciences, 2012, 111-111
ISBN
978-83-935303-0-4
Skup
XXV International Conference Genetic Days
Mjesto i datum
Wrocław, Poljska, 18.09.2012. - 20.09.2012
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
tebuconazole; prothioconazole; acetylcholinesterase; bovine erythrocytes
Sažetak
Triazole fugicides Orius 25EW® and Prosaro 250EC® (active substances: tebuconazole and respective tebuconazole plus prothioconazole)are used for the protection of wheat, barley and cole-seed against fungal diseases. Their fungicide effect is a result the inhibition of lanosterol 14-α-demetylase (CYP 51). The enzyme is included in ergosterol formation, an essential component of fungal cell membrane. Decrease of acetylcholinesterase (AChE) represents one of the important environmental contamination parameters. Inhibition of AChE causes the acetylcholine accumulation in synapses and neuromuscular plates and subsequent hyperactivation of brain and muscle tissue. AChE is localized in erythrocytes, synapses, neuromuscular plate and heart. Experiments were performed with bovine erythrocytes as a source of AChE. Changes in acetylcholinesterase activity were assessed spectrophotometrically after the exposure to Orius 25EW® and Prosaro 250EC® in vitro at concentrations of 50, 100, 150, 200, 250 and 300 µg.ml-1. Changes in absorbance were read and statistically evaluated by linear regression model (GraphPadPrism). Our results confirmed a high statistically significant inhibition of AChE activity (p < 0.001) in bovine erythrocytes exposed to both fungicides tested.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita
