Naslov
Exposure to cadmium and effects on placental steroidogenesis in rats: comparing oral versus parenteral route of exposure

Autori
Mikolić, Anja ; Kralik Oguić, Saša ; Kušec, Vesna ; Piasek, Martina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 4th Croatian Congress of Toxicology (CROTOX 2012) ; u: Arhiv za higijenu rada i toksikologiju 63 (2012) (S2) / Želježić, Davor - , 2012

Skup
4th Croatian Congress of Toxicology with the international participation

Mjesto i datum
Primošten, Hrvatska, 02.10.2012. - 05.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
cadmium exposure; placental progesterone; pregnancy; steroid disruption

Sažetak
Cadmium is a toxic environmental pollutant with potential to act as an endocrine-disrupting chemical in reproductive organs. Main sources of cadmium exposure in general population are contaminated food, water, and tobacco smoke. Up to 50% of the inhaled cadmium and an average of 5% (<1% to >20%) of cadmium oral intake is absorbed, which depends on age, nutrition, and physiological state. In exposed mammals, including humans, cadmium accumulated in placental tissue can disrupt its vital function of hormones’ synthesis during gestation. We compared effects of oral vs. parenteral cadmium route of exposure on placental steroidogenesis in laboratory rats. Previous investigation showed cadmium effects on placental progesterone production in rats (Sprague Dawley) after 19-day exposure during pregnancy at total doses of 3 or 5 mg Cd/kg b.wt sc. by osmotic pumps (Piasek et al., TEMA 10, 2000, pp. 809-12). In our new investigation, rats (Wistar) with regular four-day estrous cycle were exposed orally to 50 ppm Cd (as chloride ; 7.26±0.86 mg Cd/kg b.wt) from gestation day 1 to 20. Mother rats were then euthanized and placentas dissected, weighed, and prepared for cadmium analysis (by atomic absorption spectrometry) and steroid hormone assay (by enzyme-immunometric method). Cadmium increased in placentas of exposed rats by both routes of exposure. While parenteral cadmium exposure decreased placental progesterone, oral exposure increased progesterone concentrations in rat placentas. In conclusion, cadmium disrupts placental progesterone synthesis, which may compromise pregnancy outcome and foetal viability. This effect depends on the route of exposure during pregnancy.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

Napomena
Poster presentation P-17

POVEZANOST RADA