Naslov
Identification of candidate protein biomarkers in urine from children with idiopathic nephrotic syndrome

Autori
Kraljević Pavelić, Sandra ; Sedić, Mirela ; Gethings, Lee ; Vissers, Johannes ; Shockcor, John P. ; McDonald, Stephen ; Lemac, Maja ; Batinić, Danica ; Peter-Katalinić, Jasna ; Langridge, James

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
MSBM Summer School, Mass Spec in Biotech & Medicine

Mjesto i datum
Dubrovnik, Hrvatska, 08.07.2012. - 14.07.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Urine proteomics; biomarkers; idiopathic nephrotic syndrome

Sažetak
Idiopathic nephrotic syndrome (INS) is kidney disease characterised by several common biochemical abnormalities including massive leak of protein (albumin) into the urine (proteinuria), low blood level of albumin due to the large amounts lost in the urine (hypoalbuminemia), increased level of cholesterol in the blood (hyperlipidemia) and retention of fluid in the body (oedema) causing swelling. It accounts for about 90% of nephrosis in childhood affecting mostly children between the ages of 2 and 6 years. INS carries several serious risks for child health including the risk of kidney damage, infection (fluid that escapes the blood and enters the tissues, as happens in oedema, is susceptible to serious infection by bacteria such as E. coli), and the risk of impaired blood clotting due to loss of blood protein (albumin) that might potentially lead to pulmonary embolism, in which a blood clot cuts off blood supply to the lungs. Therefore, early diagnosis is extremely important and necessary to avoid serious pathological changes that might threaten children’s life. Since current standard clinical laboratory blood tests alone are not sufficient to detect INS with high confidence, kidney biopsy is used for its diagnosis. Novel, non-invasive diagnostic approaches are urgently needed for INS detection in children, and urine analysis might prove efficient in this respect since urine is considered an important source of biomarkers (1). In the present study, we used label-free LC-MS analysis to compare urine protein profiles of children with INS versus those from age-matched healthy children. Obtained results specifically revealed nine proteins detected only in urine from affected children including potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3, Xaa-Pro aminopeptidase 1, mitochondrial acylpyruvase FAHD1, protein phosphatase 1 regulatory subunit 1A, biliverdin reductase A, afamin, corticosteroid-binding globulin, angiotensinogen and superoxide dismutase . Additional studies on larger patient population are needed to validate the usefulness of herein identified proteins as diagnostic markers for INS in children.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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