Naslov
Evaluation of genotoxic effects of lead in pottery-glaze workers using micronucleus assay, alkaline comet assay and DNA diffusion assay

Autori
Kašuba, Vilena ; Rozgaj, Ružica ; Milić, Mirta ; Želježić, Davor ; Kopjar, Nevenka ; Pizent, Alica ; Kljaković-Gašpić, Zorana ; Jazbec, Anamarija

Izvornik
International archives of occupational and environmental health (0340-0131) 85 (2012), 7; 807-818

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
ceramic workers;
lead exposure;
biological markers;
alkaline comet assay;
micronucleus assay

Sažetak
Purpose We investigated genotoxic eVects of occupational exposure to lead acetate in pottery-glaze ceramic workers. Methods The study was carried out in 30 exposed workers and 30 matched controls, to whom several biochemical parameters—the blood lead (B-Pb ; range: exposed, 41.68– 404.77 ; controls, 12–52) and cadmium (B-Cd) level, the activity of delta-aminolevulinic acid dehydratase (ALAD), erythrocyte protoporphyrin (EP), the level of vitamin B12 and folate in serum—were measured. The genotoxic eVects were evaluated by the alkaline comet assay, the DNA diVusion assay and micronucleus test in peripheral blood lymphocytes. Results Subjects exposed to lead had signiWcantly higher B-Pb level and, consequently, increased values of tail intensity (TI), frequency of apoptotic and necrotic cells, and frequency of micronuclei (MN). In contrast, their activity of ALAD, the level of vitamin B12 and folate in serum were signiWcantly lower compared to controls. Poisson regression analysis showed a signiWcant correlation of profession, duration of exposure, smoking, level of cadmium in blood, ALAD and EP with primary DNA damage. A majority of primary damage repairs in a short period after exposure to a genotoxic agent. In addition, the inXuence of gender and level of vitamin B12 and folate in serum MN frequency in exposed group was observed. Conclusions In this study, DNA diVusion and micronucleus test showed higher inXuence of tested parameters to DNA damage. The results indicate a need for concomitant use of at least two diVerent biomarkers of exposure when estimating a genetic risk of lead exposure.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Javno zdravstvo i zdravstvena zaštita

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