Naslov
Central Croatian population data of eight X-linked markers in four linkage groups

Autori
Gršković Branka ; Mršić Gordan ; Zidkova Anastassiya ; Vrdoljak Andro ; Stenzl Vlastimil ; Popović Maja ; Primorac Dragan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
DNA in Forensics 2012 "Exploring the Phylogenies" Abstract book and Conference program / Walther Parson and Lutz Roewer - Innsbruck, 2012, 75-75

Skup
DNA in Forensics 2012 "Exploring the Phylogenies" 5th International EMPOP Meeting and 8th International Forensic Y‐User Workshop

Mjesto i datum
Innsbruck, Austrija, 06.09.2012. - 08.09.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Chromosome X; Short Tandem Repeat; Polymorphism; Population data; Kinship Testing; Central Croatia

Sažetak
The analysis of short tandem repeats (STRs) located on X chromosome shown to be particularly powerful in solving complex kinship cases. The aim of this study was to analyze 8 X-STRs in central Croatian population. We carried out a statistical analysis of the data from previously performed genetic analyses collected during routine forensic work by the Forensic Science Centre ‘‘Ivan Vučetić’’. A total of 99 unrelated healthy women and 78 men from central Croatia were typed using Mentype Argus X-8 PCR amplification kit. The allele and haplotype frequencies were determined by counting. Haplotype frequencies were calculated only in male samples. Arlequin 3.5 software was used to assess Hardy-Weinberg equilibrium (HWE), linkage disequilibrium (LD), observed and expected heterozygosity. For HWE and LD tests Bonferroni correction was used. Power of discrimination (PD) for males and females was calculated according to Desmarais. Polymorphism information content (PIC) was determined using online database ChrX-STR.org that calculates population-genetic data. In female samples deviations from HWE (p>0.00625) for each locus were not found. LD test performed on female and male samples, revealed no significant association between markers (p>0.00178). In 78 men, 37, 30, 35 and 30 haplotypes were found for linkage groups 1-4. Locus DXS10135 was the most polymorphic (PIC=0.9306). DXS7423 and DXS8378 loci showed the lowest values (0.6316 and 0.6447). PIC for whole marker set was 0.999998. PD varied from 0.6922 to 0.9345 in male and from 0.8447 to 0.9918 in female samples. Combined PD reached 99.9999% in males and 99.99999999% in females. Further analyses that will include more X-STR loci are needed to increase discrimination power for kinship and paternity testing as well as population genetics studies. Nevertheless, Mentype Argus X-8 kit can be used as an additional marker panel for forensic identification and complex kinship cases in central Croatian population.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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