Naslov
Determination of resistance and enterotoxin determinants in coagulase-negative staphylococci from dry sausages

Autori
Zdolec, Nevijo ; Filipović, Ivana ; Dobranić, Vesna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XXIII FoodMicro2012 Abstract Book / Heperkan, Dilek ; Karbancioglu-Guler, Funda ; Daskaya-Dikmen, Ceren - Istanbul : Istanbul Technical University (ITU), 2012, 655-655

ISBN
978-975-561-423-6

Skup
23rd International ICFMH Symposium FoodMicro 2012

Mjesto i datum
Istanbul, Turska, 03.09.2012. - 07.09.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
resistance genes; enterotoxin genes; staphylococci; dry sausages

Sažetak
Dominant autochthonous strains in coagulase- negative staphylococci or lactic acid bacteria population which are isolated from the specific fermented sausage should be considered as potential starter cultures for the same product. These strains are most adapted to specific intrinsic ecology of specified fermented sausage and technological procedures implemented. Selection of potential starter cultures for fermented sausages production should consider the potential hazards which are mainly related to presence of transferable antibiotic resistance markers or enterotoxins. The aim of this study was to assess the hazard potential of coagulase- negative staphylococci (CoNS) from Croatian spontaneously fermented sausages. Total of 39 isolates were collected from dry sausage “kulen” and Slavonian homemade sausage as presumptive CoNS, and subjected to ID32 Staph and PCR identification. Strains were tested for the presence of 13 enterotoxin genes ; seA, seB, seC, seG, seI, tsst1, seD, seE, seH, seJ, seM, seN and seO. Antimicrobial susceptibility of strains was tested for lincomycin, vancomycin, ampicillin, erythromycin, tetracycline, gentamycin and oxacillin by disk diffusion method and Etest. The PCR was used for detection of presence of resistance genes mecA, ermB, tetK and tetM genes. Staphylococcus epidermidis was found as dominant species (69%). All CoNS strains were negative to all enterotoxin genes. S. epidermidis showed a high rate of phenotypical resistance to tetracycline and erythromycin (44.4 % of strains). Molecular evaluation of resistance determinants revealed in eight positive S. epidermidis strains to tetK or tetM genes. Although S. epidermidis is not classical food poisoning bacteria, its presence in food could be of public health significance due possible spreading of resistance genes. Our findings implicate that spontaneous meat fermentation could result in products with potential hazard to consumers. However, more detailed and comprehensive study is needed to detect routes of transfer of resistant strains through agri-food chain. Preventing the occurrence or reducing the number of resistant commensal bacteria in animals and environment (food producing facilities) should be the prerequisite for production of safe spontaneously fermented food.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

Napomena
Prisustvovanje na kongresu i prezentiranje rezultata istraživanja omogućio je International Committee on Food Microbiology and Hygiene, stipendiranjem prvog autora.

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