Naslov
Bacteriocinogenic activity of probiotic strain L. helveticus M92

Autori
Uroić, Ksenija ; Kos, Blaženka ; Beganović, Jasna ; Leboš Pavunc, Andreja ; Šušković, Jagoda

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Skup
The international scientific conference on bacteriocins and antimicrobial peptides – BAMP2012

Mjesto i datum
Košice, Slovačka, 21.02.2012. - 23.02.2012

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antimicrobial activity; bacteriocins; induction of biosynthesis of bacteriocins; probiotics

Sažetak
Besides biopreservative potential against undesirable bacterial species in food products, bacteriocin production is often proposed to be a beneficial property of probiotic bacteria. It may contribute to colonisation resistance of the host and to protection against gastrointestinal pathogens. Bacteriocinogenic activity of Lactobacillus helveticus M92 was induced by growth in coculture with Lactococcus lactis subsp. lactis LMG 9450. Neutralized cell free supernatant (NCFS) of L. helveticus M92 obtained after cocultivation with L. lactis subsp. lactis LMG 9450 showed enhanced inhibitory effect towards indicator lactic acid bacteria strains and towards Salmonella Typhimurium FP1 strain, in comparison to the inhibitory effect of NCFS from L. helveticus M92 monoculture. Presence of bacteriocin encoding hlv gene in L. helveticus M92 was confirmed by PCR analysis. Purification of bacteriocin by gel chromatography showed that the molecular weight of antibacterial substance is higher than 5000 Da. Bacteriocin of L. helveticus M92 was not sensitive to high temperature (100°C/30 min), but was sensitive to Proteinase K treatment. Active bacteriocin fraction retained the activity during 30 days of storage at 4°C and also after vacuum drying at 40°C, which was proved to be a suitable method for obtaining bacteriocin in concentrated form without losing its activity.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA