Naslov
Preživljavanje Vibrio parahaemolyticus na niskim temperaturama in vitro
(Survival of Vibrio parahaemolyticus at low temperatures in vitro)

Autori
Skočibušić Mirjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik sažetaka priopćenja Šestog kongresa biologa Hrvatske = Proceedings of Abstracts of the Papers of the Sixth Congress of Croatian Biologists - Zagreb: Hrvatsko biološko društvo, / Huber, Đuro - Zagreb, 1997, 107-107

Skup
Šesti kongres biologa Hrvatske - The Sixth Congress of Croatian Biologists

Mjesto i datum
Opatija, Hrvatska, 22.09.1997. - 26.09.1997

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
Vibrio parahaemolyticus; nonculturable (VNC) stage; low temperature
(Vibrio parahaemolyticus; nekultivabilni stadij; niske temperature)

Sažetak
The stresses of nutrient deprivation and low temperature are to be the main causes of the induction of viable but nonculturable (VNC) stages in some pathogenic vibrios, such a Vibrio cholerae and Vibrio vulnificus. In this work, both pathogenic strain (K+) and non pathogenic starins (K-) of Vibrio parahaemolyticus were studied with regard to cell enumeration and their morphological characteristic. Cell starved at a low temperature for certain interval were counted by different enumeration methods, including plate count and direct viable count. Cell cultivability was determined by using nonselective trypticase soy agar (TSA with 3% NaCl) and highly selective thiosulfate-citrate-bile-salts-sucrose agar (TCBS). Cultures in mineral salts solution were maintained at both 5 C and room temperature (25 C) in a static state. Morphological changes of Vibrio parahaemolyticus from rods to sphere and finally became coccids was dependent on the strains and temperature at which the cells were maintained. Non cultivability cells were detected for more than 67 days at low temperature (5 C). Significant difference in time to non cultivability between cells taken from the logarithmic growth phase and cells taken from the stationary phase was observed. Preservation at room temperature for 24 h was found to eliminate the non cultivable response of cells incubated at 5 C.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA