Naslov
Analysis of 8 X-chromosomal markers in the population of Central Croatian Population

Autori
Gršković, Branka ; Zidkova, Anastassiya ; Stenzl, Vlastimil ; Popović, Maja ; Primorac, Dragan ; Mršić, Gordan

Izvornik
Croatian medical journal (0353-9504) 54 (2013), 3; 238-247

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Chromosome X; Short Tandem Repeat; Polymorphism; Population data; Kinship Testing; Central Croatia

Sažetak
Aim was to analyze 8 X-linked short tandem repeat (STR) markers in central Croatian population and to evaluate forensic efficiency of used X-STR markers. We carried out a statistical analysis of the data from previously performed genetic analyses collected during routine forensic work by the Forensic Science Centre "Ivan Vučetić". The Mentype® Argus X-8 PCR amplification kit was used for typing 99 unrelated healthy women and 78 men from central Croatia. Haplotype frequencies were calculated only in male samples. Arlequin 3.5 software was used to assess Hardy-Weinberg equilibrium (HWE), linkage disequilibrium (LD), observed (Ho) and expected heterozygosity (He). Power of discrimination (PD) for males and females, polymorphism information content (PIC), power of exclusion (PE), mean exclusion chance (MEC) for deficiency cases, normal trios and duos were determined using online database ChrX-STR.org. In female samples deviations from HWE (P>0.00625) for each locus were not found. LD test performed both on female and male samples, revealed no significant association between markers (P >0.00178). In 78 men, 37, 30, 35 and 30 haplotypes were found for four linkage groups. Locus DXS10135 was the most polymorphic (PIC=0.9306). PD varied from 0.6922 to 0.9345 in male and from 0.8447 to 0.9918 in female samples. Combined PD reached 99.999999% in males and 99.9999999999% in females. To our knowledge, this is the first population study based on X-STR analysis in Croatia. Further analyses that will include more X-chromosomal loci are needed to increase discrimination power for kinship and paternity testing as well as population genetics studies.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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