Naslov
Growth inhibition and damage of microtubule structure in neuroblastoma cell line due to radiofrequency exposure

Autori
Pavicic, Ivan ; Marjanovic, Ana Marija ; Trosic, Ivancica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
First international conference on radiation and radiation dosimetry in various fields (RAD 2012) / - , 2012, 187-187

Skup
First international conference on radiation and radiation dosimetry in various fields (RAD 2012)

Mjesto i datum
Niš, Srbija, 25.04.2012. - 27.04.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
growth; SH-SY5Y; microtubule structure; GTEM-cell; radiofrequency (RF) radiation

Sažetak
Recently published studies recognized that GSM (Global System of Mobile) modulated radiofrequency radiation (RF) may affect biological systems as a result of interference with polar intracellular macromolecular chains (1, 2). The aim of study was to evaluate whether 915 MHz radiofrequency (RF) radiation affects the microtubule cytoskeleton and/or growth of neuroblastoma cell line (SH-SY5Y). RF field was generated inside a Gigahertz Transversal Electromagnetic Mode chamber (GTEM-cell) equipped by generator, power amplifier and signal modulator. Cell samples were exposed to RF field of 915 MHz, electric field strength of 30 V/m. Average specific absorption rate (SAR) was calculated at 1.6 W/kg. Cell exposure treatment lasted for 1, 2, and 3 hours. Irradiated cell groups were matched with negative- and positive control samples. Microtubule proteins were treated immunocytochemicaly, thereafter marked by IgG anti--tubulin (SIGMA Chemical CO, St. Louis, USA) and analyzed using a fluorescent light microscope (1000x magn.). Cell growth was determined by cell counts for each hour of exposure during ten post-exposure days. In comparison with control cells, the microtubule structure clearly altered after 3 hours of irradiation (p<0.05). Significantly decreased growth was noted in cells exposed for two and three hours on third, fourth and fith day after irradiation (p<0.05). GSM modulated radiofrequency radiation (RF) may affect microtubule complex followed by growth impairment of neuroblastoma cell line SH-SY5Y.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA