Naslov
Proteome profiling of deformed vertebrae in aquarium fish black neon tetra, Hyphesobricon herbertaxelrodi L.

Autori
Horvatić, Anita ; Gjurčević, Emil ; Stanin, Damir ; Vučinić, Srđan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6th Summer Course for Mass Spectrometry in Biotechnology and Medicine / MSBM - : MSBM, 2012, 89-90

Skup
6th Summer Course for Mass Spectrometry in Biotechnology and Medicine

Mjesto i datum
Dubrovnik, Hrvatska, 07.07.2012. - 14.07.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
mass spectrometry; bone deformity; fish; proteomics

Sažetak
Fish vertebral deformities seize attention since they cause significant financial losses in fish production and processing. Factors causing skeletal disorders are reflection of expressed genotype and phenotype. Deformed fish often suffer from malnutrition and are infection prone, thus presenting potential source of infection for entire hatchery. Proteomic approach enables to set correlation of different factors with vertebral deformities at a molecular level. In this paper proteomic approach has been employed to study differentially expressed proteins in deformed and healthy fish vertebrae. Samples were analyzed by two-dimensional gel electrophoresis (2DE) and proteins were subjected to matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF/TOF). Differentially expressed proteins in healthy vertebrae are reflection of normal physiological processes related to oxidative metabolism and osteogenesis. Except calcium binding proteins, key factors in vertebrae morphogenesis are retinoid x receptors that transduce retinal signal in gene regulation. Identified proteins are promising candidates for further analyses regarding biomarker discovery.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina

POVEZANOST RADA