Naslov
Optimization of microwave-assisted extraction of phenolic compounds from broccoli and its antioxidant activity

Autori
Jokić, Stela ; Cvjetko, Marina ; Božić, Đurđica ; Fabek, Sanja ; Toth, Nina ; Vorkapić-Furač, Jasna ; Radojčić Redovniković, Ivana.

Izvornik
International journal of food science & technology (0950-5423) 47 (2012), 12; 2613-2619

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
antioxidant activity; broccoli; flavonoids; microwave-assisted extraction; phenolic compounds; response surface methodology

Sažetak
The effect of independent variables of microwave-assisted extraction, extraction temperature (50–90°C), microwave power (100–200 W), solvent concentration (methanol:water, 50–90%, v/v), and extraction time (1–27 min) on total phenolics and flavonoids contents, as well as antioxidant activity of broccoli extracts were investigated. Response surface methodology was successfully applied for optimization and the high correlation of the mathematical model indicated that a quadratic polynomial model could be used for optimization of investigated responses. Considering the maximum amount of extracted total phenolics, total flavonoids and antioxidant activity expressed as ORAC value, the optimal conditions for all three investigated responses were obtained (temperature - 71.11°C ; microwave power -167.03 W ; solvent concentration -75.95% ; extraction time 16.34 min), which is in agreement to optimal conditions obtained for each investigated responses. The development of simultaneously microwave-assisted extraction methods for total phenolics, total flavonoids, and antioxidant activity of broccoli extracts might simplify assessment of broccoli as valuables source of antioxidants.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija, Prehrambena tehnologija

POVEZANOST RADA