Naslov
Changes in wCD21+ lymphocyte subpopulation after vaccination against classical swine fever (C strain)

Autori
Terzić, Svjetlana ; Jemeršić, Lorena ; Roić, Besi ; Šandor, Ksenija

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Proceeding of the 22nd IPVS Congress / - Lahti, 2012, 488-488

ISBN
978-0620-41364-0

Skup
IPVS Congress (22 ; 2012)

Mjesto i datum
Jeju City, Republika Koreja, 10.06.2012. - 13.06.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
wCD21+ cells; classical swine fever; C strain

Sažetak
Ten crossbred pigs (12 weeks old) were vaccinated with an attenuated vaccine against CSF (C strain, 104 TCID50 of virus/dose, Veterina d.d., Zagreb, Croatia) and 10 unvaccinated pigs served as controls. Pigs belonged to the same breed and same herd. Blood samples for the leukocyte count (by Baker System 9120CP Serono Counter), differential blood count (smears by Giemsa) and single-colour flow cytometry (FCM) as well as palatine tonsilar tissue for in situ distribution of wCD21+ lymphoid cells (avidin-biotin-complex, ABC) were taken before and 28 days after vaccination1, 2. BB6-11C9 isotype of monoclonal antibodies (mAbs) for FCM analyse and immunohistochemical staining was used. The obtained data was statistically analysed by Student’s t-test was used (P<0.05). Significant differences between vaccinated and control pigs in the lymphocyte proportion at the beginning of the experiment could be explained by individual differences. The C strain did not provoke leucopenia and lymphocytopenia or the change of wCD21+ cells in peripheral blood 28 days after vaccination (Table 1). This finding may be explained by simultaneous downregulation or upregulation of T-B cells cooperation as well as it may depend of the day of sampling. In contrary, wCD21+ cells in the palatine tonsilar tissue of vaccinated pigs were scattered throughout the parafollicular area (Figure 1) more intensively than in non-vaccinated pigs (Figure 2) which is probably due to the influence of the vaccinal virus on lymphoid tissue.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA