ࡱ> FHE'` (bjbjLULU 40.?.?28 dr 2!&  } } } !!!!!!!$"h4%03!- } } 3! `!$$$ `  !$ !$$:,! P&9d v!0!Rd&hdd&!!0d&Q } + |$ d } } } 3!3!X} } } ! 222222222 Hepatic and renal sat-1 in ethylene glycol-induced oxaluria in rats Birgitta C Burckhardt1, Hrvoje Brzica2, Davorka Breljak2, Ivana Vrhovac2, Vedran Micek2, Mila Lovri3, Nina Schnedler1, Gerhard Burckhardt1, and Ivan Sabolic2 1Center of Physiology and Pathophysiology, University Medical Center, Gttingen, Germany, 2Institute for Medical Research and Occupational Health, Zagreb, Croatia, 3Clinical Institute of Laboratory Diagnosis, Zagreb, Croatia Question: The epidemiological incidence of nephrolithiasis is higher in men than in women. Most urinary stones consist of oxalate, which is largely synthesized in the liver and then released into the systemic circulation by the sulfate-oxalate exchanger, sat1. In the kidney proximal tubules, oxalate is excreted via a two-step process that includes uptake from the blood by the contraluminal sat1 and release into the urine by the luminal chloride-formate/oxalate exchanger, CFEX. The role of sat1 and CFEX in development of oxalate nephrolithiasis is unclear. Methodology: The ethylene glycol (EG) model of nephrolithiasis in male (M) and female (F) rats was used to induce oxaluric state, and to study the expression of sat1, CFEX, and rate-limiting enzymes of oxalate synthesis, alcohol dehydrogenase (Adh1) and hydroxyacid oxidase (Hao1), by real-time PCR and immunochemical methods. Result: As compared to controls, the EG-treated rats exhibited: (a) strong oxalosis and oxaluria (M>F), (b) increased abundance of sat1 protein, but not mRNA, in liver and kidneys of F rats only, (c) unchanged expression of CFEX mRNA, as well as (d) marginally changed expression of Adh1 and Hao1 mRNA in organs of both sexes. 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