Naslov
Surface Modified Liposomes by Mannosylated Conjugates Anchored via the Adamantyl Moiety in the Lipid Bilayer

Autori
Štimac, Adela ; Šegota, Suzana ; Dutour Sikirić, Maja ; Ribić, Rosana ; Frkanec, Leo ; Svetličić, Vesna ; Tomić, Srđanka ; Vranešić, Branka ; Frkanec, Ruža

Izvornik
Biochimica et biophysica acta. Biomembranes (0005-2736) 1818 (2012), 9; 2252-2259

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
adamantane ; mannose ; liposome targeting ; AFM ; DLS

Sažetak
The aim of the present study was to encapsulate mannosylated 1-aminoadamantane and mannosylated adamantyltripeptides, namely [(2R)-N-(adamant-1- yl)-3-(a, b-D-mannopyranosyloxy)-2- methylpropanamide and (2R)-N-[3-(α-D- mannopyranosyloxy)-2-methylpropanoyl]-D, L- (adamant-2-yl)glycyl-L-alanyl-D-isoglutamine] in liposomes. The characterization of liposomes, size and surface morphology was performed using dynamic light scattering (DLS) and atomic force microscopy (AFM). The results have revealed that the encapsulation of examined compounds changes the size and surface of liposomes. After the Concanavalin A (ConA) was added to the liposome preparation, increase in liposome size and their aggregation has been observed. The enlargement of liposomes was ascribed to the specific binding of the ConA to the mannose present on the surface of the prepared liposomes. Thus, it has been shown that the adamantyl moiety from mannosylated 1- aminoadamantane and mannosylated adamantyltripeptides can be used as an anchor in the lipid bilayer for carbohydrate moiety exposed on the liposome surface.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti, Biotehnologija

POVEZANOST RADA