Naslov
Standard mouse assay of antivenom quality does not measure antibodies neutralising the haemorrhagic activity of the Vipera ammodytes venom

Autori
Kurtović, Tihana ; Leonardi, Adrijana ; Lang Balija, Maja ; Brgles, Marija ; Habjanec, Lidija ; Križaj, Igor ; Halassy, Beata

Izvornik
Toxicon (0041-0101) 59 (2012), 7/8; 709-717

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Vipera ammodytes; Haemorrhagin; Ammodytoxin; Lethal toxicity assay; Lethal toxicity neutralisation; Antivenom

Sažetak
The venom of Vipera ammodytes ammodytes (Vaa), like the venoms of other Viperinae snakes, is largely haemorrhagic and necrotising, and only to a lesser extent neurotoxic to humans. The components most extensively studied so far, and most probably involved in generating the observed pathologies, are haemorrhagins (H), members of the metalloproteinase group of enzymes, and neurotoxic ammodytoxins (Atxs), that belong to the secretory phospholipases A2. Rabbit antisera were prepared containing functional antibodies specific for each class of pathology-inducing venom constituents and for both classes together. The involvement of these antibodies in neutralising the toxicity of whole Vaa venom was assessed using the ED50 assay in mice. This assay is the only regulatorily approved assay for estimating antivenom potency and as such has the task to quantify the active compound neutralising venom-induced pathology of the antivenom. Fully functional anti-Atx antibodies were shown to be responsible for neutralising the portion of venom toxicity, while anti-H antibodies were not protective in this assay. Thus, the mouse ED50 assay, intended to measure the active principle of the antivenom, does not measure antibodies specific for Vaa venom haemorrhagins, and consequently does not fulfil its primary task from the regulatory point of view.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Biotehnologija

POVEZANOST RADA