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Pregled bibliografske jedinice broj: 572182

Cortical Dynein Controls Microtubule Dynamics to Generate Pulling Forces that Position Microtubule Asters

Laan, Liedewij; Pavin, Nenad; Husson, Julien; Romet-Lemonne, Guillaume; van Duijn, Martijn; Preciado López, Magdalena; Vale, Ronald D.; Jülicher, Frank; Reck-Peterson, Samara L.; Dogterom, Marileen
Cortical Dynein Controls Microtubule Dynamics to Generate Pulling Forces that Position Microtubule Asters // Cell, 148 (2012), 3; 502-514 doi:10.1016/j.cell.2012.01.007 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 572182 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Cortical Dynein Controls Microtubule Dynamics to Generate Pulling Forces that Position Microtubule Asters

Autori
Laan, Liedewij ; Pavin, Nenad ; Husson, Julien ; Romet-Lemonne, Guillaume ; van Duijn, Martijn ; Preciado López, Magdalena ; Vale, Ronald D. ; Jülicher, Frank ; Reck-Peterson, Samara L. ; Dogterom, Marileen

Izvornik
Cell (0092-8674) 148 (2012), 3; 502-514

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
barrier-attached dynein; microtubule ends; microtubule asters

Sažetak
Dynein at the cortex contributes to microtubule- based positioning processes such as spindle positioning during embryonic cell division and centrosome positioning during fibroblast migration. To investigate how cortical dynein interacts with microtubule ends to generate force and how this functional association impacts positioning, we have reconstituted the ‘cortical’ interaction between dynein and dynamic microtubule ends in an in vitro system using microfabricated barriers. We show that barrier-attached dynein captures microtubule ends, inhibits growth, and triggers microtubule catastrophes, thereby controlling microtubule length. The subsequent interaction with shrinking microtubule ends generates pulling forces up to several pN. By combining experiments in microchambers with a theoretical description of aster mechanics, we show that dynein-mediated pulling forces lead to the reliable centering of microtubule asters in simple confining geometries. Our results demonstrate the intrinsic ability of cortical microtubule-dynein interactions to regulate microtubule dynamics and drive positioning processes in living cells.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija



POVEZANOST RADA

Projekti:
119-0982464-1253 - KSA kompjutorsko istraživanje repeticija višeg reda i duplikona u humanom genomu (Paar, Vladimir, MZOS ) ( CroRIS)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Nenad Pavin (autor)

Poveznice na cjeloviti tekst rada:

doi www.cell.com ac.els-cdn.com dx.doi.org

Citiraj ovu publikaciju:

Laan, Liedewij; Pavin, Nenad; Husson, Julien; Romet-Lemonne, Guillaume; van Duijn, Martijn; Preciado López, Magdalena; Vale, Ronald D.; Jülicher, Frank; Reck-Peterson, Samara L.; Dogterom, Marileen
Cortical Dynein Controls Microtubule Dynamics to Generate Pulling Forces that Position Microtubule Asters // Cell, 148 (2012), 3; 502-514 doi:10.1016/j.cell.2012.01.007 (međunarodna recenzija, članak, znanstveni)
Laan, L., Pavin, N., Husson, J., Romet-Lemonne, G., van Duijn, M., Preciado López, M., Vale, R., Jülicher, F., Reck-Peterson, S. & Dogterom, M. (2012) Cortical Dynein Controls Microtubule Dynamics to Generate Pulling Forces that Position Microtubule Asters. Cell, 148 (3), 502-514 doi:10.1016/j.cell.2012.01.007.
@article{article, author = {Laan, Liedewij and Pavin, Nenad and Husson, Julien and Romet-Lemonne, Guillaume and van Duijn, Martijn and Preciado L\'{o}pez, Magdalena and Vale, Ronald D. and J\"{u}licher, Frank and Reck-Peterson, Samara L. and Dogterom, Marileen}, year = {2012}, pages = {502-514}, DOI = {10.1016/j.cell.2012.01.007}, keywords = {barrier-attached dynein, microtubule ends, microtubule asters}, journal = {Cell}, doi = {10.1016/j.cell.2012.01.007}, volume = {148}, number = {3}, issn = {0092-8674}, title = {Cortical Dynein Controls Microtubule Dynamics to Generate Pulling Forces that Position Microtubule Asters}, keyword = {barrier-attached dynein, microtubule ends, microtubule asters} }
@article{article, author = {Laan, Liedewij and Pavin, Nenad and Husson, Julien and Romet-Lemonne, Guillaume and van Duijn, Martijn and Preciado L\'{o}pez, Magdalena and Vale, Ronald D. and J\"{u}licher, Frank and Reck-Peterson, Samara L. and Dogterom, Marileen}, year = {2012}, pages = {502-514}, DOI = {10.1016/j.cell.2012.01.007}, keywords = {barrier-attached dynein, microtubule ends, microtubule asters}, journal = {Cell}, doi = {10.1016/j.cell.2012.01.007}, volume = {148}, number = {3}, issn = {0092-8674}, title = {Cortical Dynein Controls Microtubule Dynamics to Generate Pulling Forces that Position Microtubule Asters}, keyword = {barrier-attached dynein, microtubule ends, microtubule asters} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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