Naslov
Diagnostic value of serum and bronchoalveolar lavage fluid levels of procalcitonin, interleukin-6 and triggering receptor expressed on myeloid cells-1 in patients with ventilator- associated pneumonia

Autori
Margetić, Sandra ; Tešija Kuna, Andrea ; Miler, Marijana ; Vrkić, Nada ; Širanović, Mladen ; Bekavac-Bešlin, Miroslav ; Mazul-Sunko, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Clinical Chemistry and Laboratory medicine / Plebani, Mario - Berlin : Walter de Gruyter, 2011, S531-S531

Skup
IFCC-WorldLab Euromedlab 2011

Mjesto i datum
Berlin, Njemačka, 15.05.2011. - 19.05.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
bronchoalveolar lavage fluid; procalcitonin; interleukin-6; ventilator-associated pneumonia

Sažetak
Background. The aim was to examine the diagnostic value of serum and bronchoalveolar lavage (BAL) fluid levels of procalcitonin (PCT), interleukin- 6 (IL-6) and triggering receptor expressed on myeloid cells-1 (TREM-1) in patients with ventilator-associated pneumonia (VAP) caused by bacteria. Methods. The study included 31 surgical intensive care unit (ICU) patients with VAP and 52 ICU patients without VAP. The concentrations of PCT, IL-6 and TREM-1 were determined in serum and BAL fluid from patients with VAP and in serum of no VAP patients. PCT was determined with an immunofluorescence assay (KRYPTOR, BRAHMS, Germany). IL-6 was measured with a chemiluminescent enzyme immunoassay (IMMULITE1000, Siemens, Germany). TREM-1 was determined with an enzyme-linked immunosorbent assay (R&D systems, Minneapolis, USA). Results. In serum, the medians of PCT, IL-6 and TREM-1 concentrations were significantly elevated in patients with VAP compared with no VAP patients: 0.680 vs 0.055 ng/mL, P<0.0001 (PCT) ; 343 vs 85 pg/mL, P=0.0006 (IL-6) ; 75.6 vs 39.5 pg/mL, P=0.0010 (TREM-1). In BAL fluid samples from patients with VAP, PCT concentrations were extremely low (0.020 ng/mL, 95%CI 0.02-0.04), IL- 6 concentrations were significantly lower than in serum (92.8 pg/mL, 95%CI 56-355, P=0.008), while TREM-1 concentrations were significantly higher than in serum (408 pg/mL, 95%CI 290-544, P<0.0001). Conclusions. Serum concentrations of PCT, IL-6 and TREM-1 have been significantly increased in patients with VAP caused by bacteria. However, in BAL fluid only TREM-1 receptor has been shown as useful in the diagnosis of VAP, suggesting bronchoalveolar TREM-1 level to be a potential marker of bacterial pulmonary infection.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Farmacija

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