Antibiotic susceptibility profiles and first report of TEM extended-spectrum β-lactamase in Pseudomonas fluorescens from coastal waters of the Kaštela Bay, Croatia

Maravić, Ana; Skočibušić, Mirjana; Šamanić, Ivica; Puizina, Jasna

doi:10.1007/s11274-012-1006-5

Pregled bibliografske jedinice broj: 569218

Antibiotic susceptibility profiles and first report of TEM extended-spectrum β-lactamase in Pseudomonas fluorescens from coastal waters of the Kaštela Bay, Croatia

Maravić, Ana; Skočibušić, Mirjana; Šamanić, Ivica; Puizina, Jasna

Antibiotic susceptibility profiles and first report of TEM extended-spectrum β-lactamase in Pseudomonas fluorescens from coastal waters of the Kaštela Bay, Croatia // World journal of microbiology & biotechnology, 28 (2012), 5; 2039-2045 doi:10.1007/s11274-012-1006-5 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 569218 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Antibiotic susceptibility profiles and first report of TEM extended-spectrum β-lactamase in Pseudomonas fluorescens from coastal waters of the Kaštela Bay, Croatia

Autori
Maravić, Ana ; Skočibušić, Mirjana ; Šamanić, Ivica ; Puizina, Jasna

Izvornik
World journal of microbiology & biotechnology (0959-3993) 28 (2012), 5; 2039-2045

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Pseudomonas fluorescens ; TEM-116 ; ESBL ; coastal waters ; RAPD

Sažetak
The aim of this study was to investigate the antibiotic susceptibility profiles and the presence of extended-spectrum-β-lactamases (ESBLs) in Pseudomonas fluorescens isolates from coastal waters of the Kaštela Bay, Croatia. Twenty-two water samples were collected during 2009. Isolates were tested for susceptibilities to 13 antibiotics by Etest. ESBL production was confirmed by double-disk synergy test carried out on Mueller-Hinton agar plates containing efflux pump inhibitor Phe- Arg-β-naphthylamide dihydrochloride. PCR and DNA sequencing analysis were used to identify ESBL-encoding genes. The transferability of cephalosporin resistance was tested by conjugation experiments. Genetic relatedness of ESBL-producing isolates was determined by random amplified polymorphic DNA (RAPD) analysis. Out of 185 P. fluorescens isolates recovered, 70 (37.8%) demonstrated multiresistance phenotype with highest rates of resistance to tetracycline (61.6%), aztreonam (31.9%), meropenem (17.3%), ceftazidime (15.1%) and cefotaxime (12.4%). Ten (5.4%) isolates were identified as ESBL producers. All isolates carried chromosomally located blaTEM-116 gene. RAPD analysis identified four different genotypes. Here, we demonstrated a baseline profiles of antimicrobial resistance of P. fluorescens from coastal waters of the Kaštela Bay, Croatia. To our knowledge, this is the first report of the presence of TEM-type ESBL in P. fluorescens, indicating this bacterium as a reservoir of antibiotic resistance genes with clinical relevance.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA

Projekti:
MZOS-177-0000000-3182 - Indikatori fekalnog onečišćenja i moguće patogene bakterije u obalnom moru (Skočibušić, Mirjana, MZOS ) ( CroRIS)
MZOS-177-1191196-0829 - Mehanizmi očuvanja stabilnosti genoma u viših biljaka (Puizina, Jasna, MZOS ) ( CroRIS)

Ustanove:
Prirodoslovno-matematički fakultet, Split

Profili:

Jasna Puizina (autor)