Naslov
First report of the golden potato cyst nematode, Globodera rostochiensis, in Bosnia and Herzegovina

Autori
Ostojić, Ivan ; Grubišić, Dinka ; Zovko, Mladen ; Miličević, Tihomir ; Gotlin Čuljak, Tanja

Izvornik
Plant disease (0191-2917) 95 (2011), 7; 883-883

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, prethodno priopćenje, znanstveni

Ključne riječi
golden potato cyst nematode; Bosnia and Herzegovina

Sažetak
The golden potato cyst nematode Globodera rostochiensis (Wollenweber, 1923) Behrens, 1975 is the most problematic pest of potato (Solanum tuberosum L. subsp. tuberosum) worldwide and it is recognized as an A1 plant - health quarantine species. In Bosna and Hercegovina there have been no surveys for the presence of potato cyst nematodes (PCN) in ware potato lands, although they have been documented in nearby countries Serbia and Croatia (3). In order to determine the presence and distribution of PCN and to prevent further spread or increase of PCN populations, a survey in ware potato land was undertaken in 2008, 2009, and 2010. Soil samples consisting of 100 cores of 4-5 ml of soil were taken in a grid pattern throughout the plots. Samples of approximately 500g were processed in the laboratory. The samples were air-dried and cysts were extracted using a Spears's flotation method (4) from a 100 ml subsample. The species identification was based on morphometrical characters (2) and real-time PCR where the ribosomal internal transcribed spacer (ITS5, PITSr3) region was amplified and detected by the Sequence Dection Software v1.3 (Applied Biosystems) (1). In 2008, soil sampling was conducted in five locations and PCN were detected in any of ten soil samples taken. In June 2009 a total of 17 soil samples were collected from seven locations. Two viable cysts of G. rostochiensis were detected in one soil sample from Tihaljina (Zaside ; E 6447659.560, N 4797392.101, altitude 130 m). A total of 110 soil samples were collected in 90 locations in May and June 2010. Five viable cysts of G. rostochiensis were detected in one soil sample taken from Čapljina (Opličići – Turajlovina ; E 6485169, N 4775964, altitude 222 m). Based on survey results, G. rostochiensis is not widely spread but the cysts are detectable, indicating that the infestation is relatively recent and that there is a threat of further spread of this quarantine species. Consequently, producers need to grow resistant potato cultivars and implement additional control measures in order to keep the nematode populations below damaging levels.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA