Naslov
Determination of thiamine, riboflavin, niacin and pyridoxine in flours made from organically and conventionally grown wheat

Autori
Bojić, Mirza ; Mendaš, Gordana ; Vinković-Vrček, Ivana ; Šarića, Darija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7. Encontro Nacional de Cromatografia : sumários / Barros, Aquiles A ; et al. - Porto : Sociedade Portuguesa de Química, 2012, 174-174

ISBN
978-989-97667-0-9

Skup
Encontro Nacional de Cromatografia (7 ; 2012)

Mjesto i datum
Porto, Portugal, 09.01.2012. - 11.01.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
flour; vitamins

Sažetak
Introduction. Wheat flour presents rich source of vitamins: thiamine, riboflavin and pyridoxine ; moreover B vitamins have high bioavailability from cereal products. Objective of this work was to compare content of pyridoxine, thiamine, niacin and riboflavin in flour from organically vs. conventionally grown wheat. Materials and methods. 5 g of flour was homogenized with 20 g of ultrapure water for 30 minutes. The mixture was centrifuged for 30 min at 4000 g and 10 mL of supernatant was used for solid phase extraction (SPE) on Sep-Pak C18 columns. HPLC analysis was conducted as described by Ekinci and Kadakal [1]. Detection was based on retention time and DAD spectra. Quantification was performed using appropriate calibration curves. Results. A total of 28 flour samples were analyzed (7 cultivars/breeding lines ; each organically and conventionally grown in 2008 and 2009). Pyridoxine was quantified in 10 samples (0.054 - 0.204 μg/g), niacin in 6 samples (0.422 - 1.825 μg/g), thiamine in 5 samples (0.062 - 0.748 μg/g) and riboflavin in 2 samples (0.080 - 1.232 μg/g). It should be noted that total values of these water soluble vitamins are generally lower compared to the results reported [2] as neither hydrochloric acid hydrolysis, nor enzymatic treatment was used in sample preparation. Conventionally grown wheat is generally richer in vitamin content compared to organically grown. Better yield of vitamins in conventionally grown wheat is not surprising as conventionally grown wheat was fertilized (N 145 kg/ha, P2O5 80 kg/ha and K2O 120 kg/ha). Conclusion. Pyridoxine, thiamine and niacin were detected in both conventionally and organically grown wheat. Pyridoxine was the most commonly found vitamin while thiamine was found in highest concentrations. Generally flour from conventionally grown wheat is richer in analyzed vitamins.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA