Pregled bibliografske jedinice broj: 560570
Immunobiology of recombinant CMV Strain expressing ligand for NKG2D receptor - a dramatic attenuation in vivo does not prevent an efficient and long-lasting protective immune response
Immunobiology of recombinant CMV Strain expressing ligand for NKG2D receptor - a dramatic attenuation in vivo does not prevent an efficient and long-lasting protective immune response // 12th meeting of the Society for natural immunity and NK 2010, 11.-15. September 2010 Cavtat-Dubrovnik, Book of abstracts / / Jonjić, Stipan ; Krmpotić, Astrid ; Watzl, Carsten (ur.). (ur.).
Rijeka, 2010. str. 78-78 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 560570 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Immunobiology of recombinant CMV Strain expressing ligand for NKG2D receptor - a dramatic attenuation in vivo does not prevent an efficient and long-lasting protective immune response
Autori
Slavuljica, Irena ; Busche, Andreas ; Babić, Marina ; Mitrović, Maja ; Gašparović, Iva ; Cekinović, Đurđica ; Markova Car, Elitza ; Pernjak Pugel, Ester ; Ciković, Ana ; Juranić Lisnić, Vanda ; Messerle, Martin ; Krmpotić, Astrid ; Jonjić, Stipan.
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
12th meeting of the Society for natural immunity and NK 2010, 11.-15. September 2010 Cavtat-Dubrovnik, Book of abstracts /
/ Jonjić, Stipan ; Krmpotić, Astrid ; Watzl, Carsten (ur.). - Rijeka, 2010, 78-78
Skup
12th meeting of the Society for natural immunity and NK 2010,
Mjesto i datum
Dubrovnik, Hrvatska; Cavtat, Hrvatska, 11.09.2010. - 15.09.2010
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
MCMV; NKG2D; RAE-1gamma;
Sažetak
Cytomegalovirus (CMV) is a major viral cause of morbidity and mortality after congenitaly infected and immunocompromised individuals, such as transplant recipiens and HIV-infected pacients. This is why developing an effective and safe HCMV vaccine is utmost importance. NKG2D is a potent activating receptor expressed by the cells of innate and adaptive immunity. Its importance in CMV immunesurveillance is indicated by the elaborative viral evasion mechanisms evolved to avoid NKG2D. In order to study this powerful signaling pathway we designed a recombinant mouse CMV expressing the high affinity NKG2D ligand RAE-1γ (RAE-1γMCMV). Expression of RAE-1γ by MCMV resulted in profound virus attenuation in vivo and lower latent virus DNA loads. RAE-1γMCMV infection was efficiently controlled by immunodeficient hosts, including mice lacking type I interferons receptor and mice immunosuppressed by sublethal γ-irradiation. Features of neonatal RAE-1γMCMV infection were also diminished. Despite tight innate immune control, RAE-1γMCMV infection elicited strong, long-lasting protective immunity. Moreover, upon maternal vaccination with MCMV expressing NKG2D ligand, transplacental transfer of antiviral antibodies protected neonatal mice from CMV disease. RAE-1γ transgene exhibited no sequence variation throughout infection despite of selective pressure. Live, attenuated viruses have large potential to serve as a vaccine vectors. The capacity of recombinant RAE-1γMCMV to serve as a vaccine vector for other viral and bacterial pathogens is currently under investigation. Altogether, our results indicate that a recombinant virus encoding a ligand for NKG2D receptor might provide a new, powerful approach for development of a safe and immunogenic CMV vaccine as well as CMV-based vaccine vector.
Izvorni jezik
Engleski
POVEZANOST RADA
Projekti:
062-0621261-1263 - Molekularni mehanizmi citomegalovirusnog izmicanja imunološkom nadzoru (Jonjić, Stipan, MZOS ) ( CroRIS)
062-0621261-1268 - Uloga imunosubverzivnih citomegalovirusnih gena u latenciji (Krmpotić, Astrid, MZOS ) ( CroRIS)
062-0621261-1269 - Perinatalni citomegalovirusni encefalitis (Pernjak-Pugel, Ester, MZOS ) ( CroRIS)
Ustanove:
Medicinski fakultet, Rijeka
Profili:
Iva Gašparović-Curtini (autor)
Elitza Petkova Markova Car (autor)
Astrid Krmpotić (autor)
Ester Pernjak-Pugel (autor)
Đurđica Cekinović Grbeša (autor)
Vanda Juranić Lisnić (autor)
Irena Slavuljica (autor)
Maja Arapović (autor)
Marina Babić Čač (autor)
Stipan Jonjić (autor)
Ana Ciković (autor)