Naslov
Determination of enzyme matrix metalloproteinases-9 and immune status as indicators of development of the environmental diseases

Autori
Bulog, A ; Mićović, V ; Šuljić, P ; Mrakovčić-Šutić, I

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik radova 4. međunarodnog kongresa hrvatskog društva "Albert Einstein" / - , 2011

Skup
4th INTERNATIONAL CONGRESS OF CROATIAN SOCIETY“ALBERT EINSTEIN“

Mjesto i datum
Rab, Hrvatska, 26.05.2011. - 28.05.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
environmental diseases; innate immunity; matrix metalloproteinases 9

Sažetak
The majority of environmental diseases are multifactorial airway illnesses, including genetic background and exposure to different kind of airborne irritants and allergens. Altered lifestyle and changes in environmental exposures (such as: traffic emissions, cigarette smoke, photochemical smog components, industry irritants) contribute to the occurring of these diseases. The term of environmental illnesses includes the disease primarily caused by pollution of air and water, chemical and physical agents, radiation, contaminated food and direct contact with the toxins we are exposed to natural and/or working environment. The members of the matrix metalloproteinase (MMP) family are involved in the pathogenesis of COPD. MMPs comprise a large family of structurally related zinc metalloendopeptidases with different substrate specificities and possibilities to degrade protein constituents of the extracellular matrix. Subjects and methods: We investigated immunological status and level of MMP-9 in workers occupationally exposed to volatile aromatic hydrocarbons (shipyard Viktor Lenac in Rijeka and Kraljevica in Kraljevica) compared to urban residents (Rijeka) and rural areas (Mali Lošinj). The phenotypic profiles of peripheral blood lymphocytes were done by flow cytometry. The method of enzyme immunoassay (ELISA) was used to determine enzyme expression of matrix metalloproteinase-9 (MMP-9). Statistical data were calculated using computer program Statistica 7.1. Results: The occupationally exposed group had a statistically significantly elevated level of MMP-9 in the urine, and the percentage of cells of innate immunity was significantly increased. Conclusion: Our results showed a large increase in the enzyme MMP-9 in the urine of workers occupationally exposed to volatile aromatic hydrocarbons, accompanied by growth of cells of innate immunity (NKT and T regulatory cells) in peripheral blood, which could contribute to the monitoring, early detection of environmental diseases and consequent earlier and more effective treatment.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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