Naslov
The analysis of enzyme matrix metalloproteinases-9 and immune status in workers occupationally exposed to volatile aromatic hydrocarbons

Autori
Mrakovčić-Šutić, Ines ; Bulog, Aleksandar ; Mićović, Vladimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik radova međunarodnog susreta Hrvatskog društva fiziologa / - , 2011

Skup
International Meeting of Croatian Physiological Society

Mjesto i datum
Osijek, Hrvatska, 23.09.2011. - 25.09.2011

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
environmental diseases; matrix metalloproteinases 9; work occupated diseases

Sažetak
Introduction: The majority of environmental diseases are multifactorial airway illnesses, including genetic background and exposure to different kind of airborne irritants and allergens. Altered lifestyle and changes in environmental exposures contribute to the occurring of these diseases. The term of environmental illnesses includes the disease primarily caused by pollution of air and water, chemical and physical agents, radiation, contaminated food and direct contact with the toxins we are exposed to natural and/or working environment. The members of the matrix metalloproteinase (MMP) family are involved in the pathogenesis of COPD. MMPs comprise a large family of structurally related zinc metalloendopeptidases with different substrate specificities and possibilities to degrade protein constituents of the extracellular matrix. Patients and methods: We performed in workers occupationally exposed to volatile aromatic hydrocarbons (shipyard Viktor Lenac in Rijeka and Kraljevica in Kraljevica) compared to urban residents (Rijeka) and rural areas (Mali Lošinj). Assessment of immune status were done by flow cytometry and determined the phenotypic association with peripheral blood mononuclear cells, intracellular staining and cytolytic potential of these cells. The method of enzyme immunoassay (ELISA) were used to determine enzyme expression of matrix metalloproteinase-9 (MMP-9) and gas chromatography for determination of BTEX (benzene, toluene, m / p xylene and o-xylene). Statistical data were calculated using computer program Statistica 7.1. Results: The occupationally exposed group had a significantly elevated level of enzyme MMP-9 in the urine, accompanied with augmentation of cells of innate immunity (NKT and T regulatory cells), in peripheral blood. Conclusion: Our results showed a large increase in the enzyme MMP-9 and BTEX in the urine of workers occupationally exposed to volatile aromatic hydrocarbons, accompanied by growth of cells of innate immunity in peripheral blood, which could contribute to the monitoring, early detection of environmental diseases and consequent earlier and more effective treatment.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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