Naslov
UTJECAJ RADIOIZOTOPA 201Tl I 99mTc PRIMIJENJENIH U DIJAGNOSTIĈKE SVRHE NA RAZINU OŠTEĆENJA GENOMA U LIMFOCITIMA PERIFERNE KRVI – PRIKAZ SLUĈAJA
(PERIPHERAL BLOOD LYMPHOCYTE GENOME DAMAGE INDUCED BY DIAGNOSTIC EXPOSURE TO 201Tl AND 99mTc: A CASE STUDY)

Autori
Kopjar, Nevenka ; Ramić, Snježana ; Željezić, Davor ; Prlić, Ivica

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), ostalo

Izvornik
Zbornik radova VIII. simpozija Hrvatskog društva za zaštitu od zračenja s međunarodnim sudjelovanjem / Krajcar Bronić, Ines ; Kopjar, Nevenka ; Milić, Mirta ; Branica Gina - Zagreb : Hrvatsko društvo za zaštitu od zračenja, 2011, 134-141

ISBN
978-953-96133-7-0

Skup
VIII. simpozij Hrvatskog društva za zaštitu od zračenja s međunarodnim sudjelovanjem

Mjesto i datum
Krk, Hrvatska, 13.04.2011. - 15.04.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
na

Sažetak
The levels of genome damage induced by diagnostic exposure to radioisotopes 201Tl and 99mTc were studied in human peripheral blood leukocytes using the alkaline comet assay, the analysis of structural chromosome aberrations (CA) and cytokinesis-block micronucleus (MN) assay. A subject of study was an engineer occupationally exposed to ionising and nonionising radiation for about 25 years. Due to health problems, he has been advised to perform two nuclear medicine cardiac imaging procedures. In the first one he was given 201Tl (activity 111 MBq), while second procedure involved the exposure to 99mTc (activity 740 MBq). In the course of study (21 day) radiation doses were monitored using both active electronic, and passive film badge dosimeters. Venous blood was collected before and after administration of 201Tl, before and after administration of 99mTc, and 7 days after second cardiac imaging procedure. Although the subject received two high radiation doses in a short time period, results of the alkaline comet assay suggest that the levels of primary DNA damage in leukocytes were not significantly impaired. The highest level of CA was found in the blood sample collected after administration of 201Tl, and moderate levels after administration of 99mTc. The highest incidence of MN was observed in 3rd blood sample collected before administration of 99mTc, which was in line with the mechanisms of MN formation following exposure to ionising radiation. Low levels of primary DNA damage observed in the course of study, along with low levels of CA and MN found in 5th blood sample point to effective repair mechanisms but also suggest the existence of an adaptive response in a subject, possibly induced by his long-term occupational exposure to low doses of both ionising and nonionising radiation.

Izvorni jezik
Hrvatski

Znanstvena područja
Fizika, Biologija, Javno zdravstvo i zdravstvena zaštita

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