Naslov
Proinflammatory role of dipeptidyl peptidase IV in patients with chronic obstructive pulmonary disease

Autori
Somborac, Anita ; Pancirov, Dolores ; Ostojić, Sunčica ; Detel, Dijana ; Varljen, Jadranka ; Čepelak, Ivana ; Žanić Grubišić, Tihana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FEBS Journal: Abstracts of the 36th FEBS Congress ''Biochemistry for Tomorrow's Medicine'' / Perham, Richard - Oxford : Wiley-Blackwell, 2011, 305-305

Skup
36th FEBS Congress ''Biochemistry for Tomorrow's Medicine''

Mjesto i datum
Torino, Italija, 25.06.2011. - 30.06.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
dipeptidyl peptidase IV ; chronic obstructive pulmonary disease ; C-reactive protein ; inflammation

Sažetak
Background: Chronic obstructive pulmonary disease (COPD) is a respiratory disease characterised by airflow limitation and abnormal inflammatory response of lungs to noxious particles and gases. Chronic inflammation is associated with the infiltration of immune cells which release proinflammatory mediators into the lung. Beside a local inflammatory process, COPD patients are also showing systemic inflammation. Dipeptidyl peptidase IV (DPP IV) is a membrane-bound glycoprotein present at the surface of most cell types, including lymphocytes, with a known activity in serum. DPP IV is a serine exopeptidase which selectively removes X-Pro dipeptide from different proinflammatory neuropeptides, chemokines, cytokines and other biomolecules, thus contributing to their functional activation or inactivation. Our goal was to correlate DPP IV activity with classic markers of inflammation in COPD patients. Materials and Methods: We obtained blood and serum samples from COPD patients (N=106) and healthy volunteers (N=38). DPP IV activity was determined using the spectrophotometric method with a glicyl-prolyl-p-nitroanilid as substrate. C-reactive protein (CRP) concentration was measured by immunoturbidimetric assay. Total leukocyte number and proportions of neutrophils and lymphocytes were determined using the Cell-Dyn 3200 hematology analyzer. Results: DPP IV activity and percentage of lymphocytes are significantly reduced, while CRP concentration, total leukocyte number and percentage of neutrophils are significantly higher in COPD patients comparing to healthy controls (P<0, 001). Furthermore, DPP IV activity is showing weak correlation with the percentage of lymphocytes (r=0, 355, P<0, 001) and weak inverse correlation with the CRP concentration (r=-0, 340, P<0, 001) and total leukocyte number (r=-0, 367, P<0, 001). Conclusions: Decreased activity of DPP IV found in COPD patients might be a key factor contributing to the development of both local and systemic inflammation in COPD.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija

POVEZANOST RADA