Naslov
Signaling pathways in human acute myeloid leukemia

Autori
Perkovic, Sanja ; Batinic, Josip ; Dubravcic, Klara ; Batinic, Drago

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Cytometry Part B (Clinical Cytometry) 2011 ; 80B / - , 2011, 405-405

Skup
11th Euroconference on Clinical Cell Analysis

Mjesto i datum
Dublin, Irska, 13.09.2011. - 17.09.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
acute myeloid leukemia; signaling pathways

Sažetak
The aim of our research was to determine phosphorylation status of leukemic blasts by flow cytometry. In this study, we stained cells with anti-AKT (pT308), anti-ERK1/2 (pT202/pY204), and anti-p38 MAPK (pT180/pY182) monoclonal antibodies. In addition, we stained cells with anti-CD45 and/or anti-CD34 to gate only blasts in our samples. Kolmogorov-Smirnov statistical test was used to compare expression of phosphorylated molecules to negative isotype control staining. D value greater than 0.2 (D > 0.2) was considered positive. We found Akt phosphorylation/ activation in 30/48 (62.5%), ERK1/2 in 25/48 (52.1%), and p38 MAPK in 18/48 (37.5%) AML samples (in concordance with results of other AML phosphorylation studies). Interestingly, of these 18 p38 MAPK positive cases, 16 (88.8%) were also Akt positive. Statistical analysis revealed significant correlation (P ¼ 0.0001 ; r ¼ 0.55) between Akt and p38 phosphorylation, and chi-square test showed significantly higher frequency of AKT activation in p38 phosphorylated samples (P ¼ 0.009). In conclusion, signal transduction pathways PI3K/Akt and MAPK are dysregulated in human AML. Activation of p38 accompanied by Akt activation could indicate possible interactions and crosstalk between two signaling cascades. However, this hypothesis remains to be elucidated by further experiments.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA