Pregled bibliografske jedinice broj: 55160
Biological Repair of Thyroid Cartilage Defects by Osteogenic Protein-1 (Bone Morphogenetic Protein-7) in Dog
Biological Repair of Thyroid Cartilage Defects by Osteogenic Protein-1 (Bone Morphogenetic Protein-7) in Dog // Growth factors, 17 (2000), 221-232 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 55160 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Biological Repair of Thyroid Cartilage Defects by Osteogenic Protein-1 (Bone Morphogenetic Protein-7) in Dog
(Biological Repair of Thyroid Cartilage Defects by Osteogenic Protein-1 (Bone Morphogenetic Protein-1) in Dog)
Autori
Katić, Vladimir ; Majstorović, Lidija ; Matičićc, Dražen ; Pirkić, Boris ; Yin, Samuel ; Kos, Josip ; Martinović, Snježana ; McCartney, John E. ; Vukičević, Slobodan
Izvornik
Growth factors (0897-7194) 17
(2000);
221-232
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
osteogenic protein-1; bone morphogenetic protein-7; cartilage repair; dog larynx
(osteogenic protein-1; bone morphogenetic protein-7; cartilage repai; dog larynx)
Sažetak
The efficacy of human recombinant osteogenic protein-1 (OP-1, bone morphogenetic protein-7) in regeneration of dog larynx was examined by treating thyroid cartilage defects (1.5 cm2) in dogs with thyroid allografts covered with host perichondrium or fascia. Prior to implantation allografts were frozen, thawed and demineralized. The treatment groups were as follows: I - Allograft control implant (n=3) II - Implants coated with 500 ug OP-1 (n=4) III - Implants coated with 100 ug OP-1 (n=3) IV - Implants coated with 500 ug OP-1 and covered with neck fascia (n=3), and V - Implants extracted with 1 M NaCl and guanidine hydrochloride, and coated with 500 ug OP-1 (n=4). Dogs were sacrificed four months following surgery. Each larynx was removed, carefully dissected and a three-dimensional reconstruction of the defect area was performed on serial sections. The results revealed that the implants of control dogs remained intact with no apparent reduction in size and new tissue formation. OP-1 enriched thyroid allografts, dose dependently induced bone, cartilage and ligament-like structures comprising up to 80% of the total regenerated defect area. Boundaries of the defects healed by formation of new bone when bone resided within the old thyroid cartilage layers. Old cartilage not containing bone within its layers healed by complete integration with newly formed cartilage. Both new bone and cartilage were embedded into layers of new ligament-like tissue which expressed specific morphologic and molecular markers. The three newly formed tissues were tightly connected into a "bone-cartilage-ligament continuum" of tissues, suggesting that OP-1 served as a multiple tissue morphogen in this specific mocroenvironment.
Izvorni jezik
Engleski
Znanstvena područja
Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
108103
Ustanove:
Medicinski fakultet, Zagreb
Profili:
Vladimir Katić
(autor)
Slobodan Vukičević
(autor)
Boris Pirkić
(autor)
Snježana Martinović
(autor)
Dražen Matičić
(autor)
Josip Kos
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE