Naslov
Antioxidant enzyme status in nod mouse embryos during diabetic pregnancy
(Antioxidant enzyme status in nod mouse embryos during diabetic pregnancy MOUSE EMBRYOS DURING DIABETIC PREGNANCY)

Autori
Korolija, Marina ; Popović Hadžija, Marijana ; Sobočanec, Sandra ; Mačak Šafranko, Željka ; Hadžija, Mirko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
The 6th international symposium on diabetes and pregnancy : book of abstracts / - Salzburg, 2011, 1-1

Skup
International symposium on diabetes and pregnancy (6 ; 2011)

Mjesto i datum
Salzburg, Austrija, 23.03.2011. - 26.03.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
diabetic embryopathy; oxidative stress; neural tube defects; non-obese diabetic mice

Sažetak
Maternal diabetes increases the rate of congenital malformations in embryos, causing diabetic embryopathy which mostly affects heart and the neural tube. Although the cellular and molecular mechanisms of diabetic embryopathy are poorly understood, it was shown that maternal hyperglycemia induces oxidative stress in embryos. We employed non-obese diabetic mouse strain as an animal model in order to evaluate the incidence of neural tube defects (NTDs) and establish whether or not the antioxidant enzyme status in embryos changes during diabetic pregnancy. We compared E (embryonic day) 10.5 embryos from non-diabetic and alloxan-induced diabetic pregnancies. We assayed mRNA and protein expression, as well as the activity of major antioxidant enzymes that scavenge superoxide (CuZn-superoxide dismutase - Sod1, Mn-superoxide dismutase - Sod2) and catabolize subsequently produced hydrogen peroxide (catalase – Cat, glutathione peroxidase 1 - Gpx1). Methods: Gene expression was analyzed by real-time PCR. Protein quantification was performed using SDS-PAGE and Western blotting. Enzyme activities were determined spectrophotometrically. According to external morphology, we found exencephaly as the most common NTD, affecting nearly 10% of all embryos developed under high glucose conditions. Compared to embryos of healthy mothers, no alteration in mRNA content of sod1, sod2, cat and gpx1 was detected. However, significant increase in Sod2 and Gpx1 protein level and activity was observed. Antioxidant enzyme gene expression does not reflect oxidative stress, while quantity and activity changes in antioxidant enzymes indicate excessive reactive oxygen species production and increased demand for its detoxification in embryos exposed to maternal diabetes.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA