Naslov
The phenanthridine biguanides efficiently differentiate between dGdC, dAdT and rArU sequences by two independent, sensitive spectroscopic methods

Autori
Piantanida, Ivo ; Radić Stojković, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
47th International Conference on Medicinal Chemistry : Drug Discovery and Selection

Mjesto i datum
Lyon, Francuska, 06.07.2011. - 08.07.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
phenanthridine; DNA; RNA; fluorescence; CD

Sažetak
At submicromolar concentrations two novel phenanthridine biguanides efficiently differentiate between dGdC and dAdT sequences by opposite fluorimetric changes (quenching for dGdC and increase for dAdT) and especially bis- biguanide derivative gives opposite ICD response (negative ICD for dGC and strong positive for dAdT). This specific signalling was explained by ability of compounds to switch binding mode from intercalation into dGdC to minor groove binding into dAdT sequences. Both compounds bind to rArU by intercalation, yielding different fluorimetric and CD response in comparison to any ds-DNA. Moreover, both compounds revealed significantly higher affinity toward ds-polynucleotides in comparison to previously studied alkylamine- and urea-analogues.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA