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Pregled bibliografske jedinice broj: 549329

Chondrogenic potential of human synovial mesenchymal stem cells in alginate


Kurth, T.; Hedbom, E.; Shintani, N.; Sugimoto, M.; Chen, F.H.; Hašpl, Miroslav; Martinović, Snježana; Hunziker, E.B.
Chondrogenic potential of human synovial mesenchymal stem cells in alginate // Osteoarthritis and cartilage, 15 (2007), 10; 1178-1189 doi:10.1016/j.joca.2007.03.015 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 549329 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Chondrogenic potential of human synovial mesenchymal stem cells in alginate

Autori
Kurth, T. ; Hedbom, E. ; Shintani, N. ; Sugimoto, M. ; Chen, F.H. ; Hašpl, Miroslav ; Martinović, Snježana ; Hunziker, E.B.

Izvornik
Osteoarthritis and cartilage (1063-4584) 15 (2007), 10; 1178-1189

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
human synovial mesenchymal stem cells; chondrogenic potential

Sažetak
In a recent study, we demonstrated that mesenchymal stem cells (MSCs) derived from the synovial membranes of bovine shoulder joints could differentiate into chondrocytes when cultured in alginate. The purpose of the present study was to establish the conditions under which synovial MSCs derived from aging human donors can be induced to undergo chondrogenic differentiation using the same alginate system. MSCs were obtained by digesting the knee-joint synovial membranes of osteoarthritic human donors (aged 59-76 years), and expanded in monolayer cultures. The cells were then seeded at a numerical density of 4x10(6)/ml within discs of 2% alginate, which were cultured in serum-containing or serum-free medium (the latter being supplemented with 1% insulin, transferrin, selenium (ITS). The chondrogenic differentiation capacity of the cells was tested by exposing them to the morphogens transforming growth factor-beta1 (TGF-beta1), TGF-beta2, TGF-beta3, insulin-like growth factor-1 (IGF-1), bone morphogenetic protein-2 (BMP-2) and BMP-7, as well as to the synthetic glucocorticoid dexamethasone. The relative mRNA levels of collagen types I and II, of aggrecan and of Sox9 were determined quantitatively by the real-time polymerase chain reaction (PCR). The extracellular deposition of proteoglycans was evaluated histologically after staining with Toluidine Blue, and that of type-II collagen by immunohistochemistry. BMP-2 induced the chondrogenic differentiation of human synovial MSCs in a dose-dependent manner. The response elicited by BMP-7 was comparable. Both of these agents were more potent than TGF-beta1. A higher level of BMP-2-induced chondrogenic differentiation was achieved in the absence than in the presence of serum. In the presence of dexamethasone, the BMP-2-induced expression of mRNAs for aggrecan and type-II collagen was suppressed ; the weaker TGF-beta1-induced expression of these chondrogenic markers was not obviously affected. We have demonstrated that synovial MSCs derived from the knee joints of aging human donors possess chondrogenic potential. Under serum-free culturing conditions and in the absence of dexamethasone, BMP-2 and BMP-7 were the most potent inducers of this transformation process.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
108-1080327-0161 - Liječenje bolesti i ozljeda hrskavice velikih zglobova (Hašpl, Miroslav, MZOS ) ( CroRIS)

Ustanove:
Medicinski fakultet, Zagreb

Profili:

Avatar Url Snježana Martinović (autor)

Avatar Url Miroslav Hašpl (autor)

Poveznice na cjeloviti tekst rada:

doi www.oarsijournal.com

Citiraj ovu publikaciju:

Kurth, T.; Hedbom, E.; Shintani, N.; Sugimoto, M.; Chen, F.H.; Hašpl, Miroslav; Martinović, Snježana; Hunziker, E.B.
Chondrogenic potential of human synovial mesenchymal stem cells in alginate // Osteoarthritis and cartilage, 15 (2007), 10; 1178-1189 doi:10.1016/j.joca.2007.03.015 (međunarodna recenzija, članak, znanstveni)
Kurth, T., Hedbom, E., Shintani, N., Sugimoto, M., Chen, F., Hašpl, M., Martinović, S. & Hunziker, E. (2007) Chondrogenic potential of human synovial mesenchymal stem cells in alginate. Osteoarthritis and cartilage, 15 (10), 1178-1189 doi:10.1016/j.joca.2007.03.015.
@article{article, author = {Kurth, T. and Hedbom, E. and Shintani, N. and Sugimoto, M. and Chen, F.H. and Ha\v{s}pl, Miroslav and Martinovi\'{c}, Snje\v{z}ana and Hunziker, E.B.}, year = {2007}, pages = {1178-1189}, DOI = {10.1016/j.joca.2007.03.015}, keywords = {human synovial mesenchymal stem cells, chondrogenic potential}, journal = {Osteoarthritis and cartilage}, doi = {10.1016/j.joca.2007.03.015}, volume = {15}, number = {10}, issn = {1063-4584}, title = {Chondrogenic potential of human synovial mesenchymal stem cells in alginate}, keyword = {human synovial mesenchymal stem cells, chondrogenic potential} }
@article{article, author = {Kurth, T. and Hedbom, E. and Shintani, N. and Sugimoto, M. and Chen, F.H. and Ha\v{s}pl, Miroslav and Martinovi\'{c}, Snje\v{z}ana and Hunziker, E.B.}, year = {2007}, pages = {1178-1189}, DOI = {10.1016/j.joca.2007.03.015}, keywords = {human synovial mesenchymal stem cells, chondrogenic potential}, journal = {Osteoarthritis and cartilage}, doi = {10.1016/j.joca.2007.03.015}, volume = {15}, number = {10}, issn = {1063-4584}, title = {Chondrogenic potential of human synovial mesenchymal stem cells in alginate}, keyword = {human synovial mesenchymal stem cells, chondrogenic potential} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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