Naslov
Inferring spatial organization of bonds within adhesion clusters by exploiting fluctuations of soft interfaces

Autori
Smith, Ana-Sunčana ; Fenz, Susanne Franzoska ; Sengupta, Kheya

Izvornik
Europhysics Letters (0295-5075) 89 (2010), 2; 28003-1

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
adhesion ; membrane

Sažetak
Detecting the organization of bonds within adhesion domains connecting two interacting membranes is, at present, extremely challenging. Herein we present a technique, based on Reflection Interference Contrast Microscopy, which uses spontaneous thermal fluctuations of a soft interface as a tool to identify the organization of specific ligand-receptor bonds. The key is a time-resolved analysis of micro-interferometric data that systematically quantifies fluctuations and enables the detection of their suppression due to the formation of bonds, which, in turn, allows the identification of the bond organization without the use of fluorescent labelling. The identification of a new type of bond organization characterized by sparsely distributed bonds, as well as detection of pinning centres of nanometric size is presented.

Izvorni jezik
Engleski

Znanstvena područja
Fizika

POVEZANOST RADA